Controlled Nutrient Delivery to Pancreatic Islets Using Polydopamine-Coated Mesoporous Silica Nanoparticles

Mehdi Razavi; Rosita Primavera; Bhavesh D. Kevadiya; Jing Wang; Mujib Ullah; Peter Buchwald; Avnesh S. Thakor

doi:10.1021/acs.nanolett.0c02576

Controlled Nutrient Delivery to Pancreatic Islets Using Polydopamine-Coated Mesoporous Silica Nanoparticles

Mehdi Razavi, Rosita Primavera, Bhavesh D. Kevadiya, Jing Wang, Mujib Ullah, Peter Buchwald, Avnesh S. Thakor

Molecular and Cellular Pharmacology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In the present study, we created a nanoscale platform that can deliver nutrients to pancreatic islets in a controlled manner. Our platform consists of a mesoporous silica nanoparticle (MSNP), which can be loaded with glutamine (G: an essential amino acid required for islet survival and function). To control the release of G, MSNPs were coated with a polydopamine (PD) layer. With the optimal parameters (0.5 mg/mL and 0.5 h), MSNPs were coated with a layer of PD, which resulted in a delay of G release from MSNPs over 14 d (57.4 ± 4.7% release). Following syngeneic renal subcapsule islet transplantation in diabetic mice, PDG-MSNPs improved the engraftment of islets (i.e., enhanced revascularization and reduced inflammation) as well as their function, resulting in re-establishment of glycemic control. Collectively, our data show that PDG-MSNPs can support transplanted islets by providing them with a controlled and sustained supply of nutrients.

Original language	English (US)
Pages (from-to)	7220-7229
Number of pages	10
Journal	Nano Letters
Volume	20
Issue number	10
DOIs	https://doi.org/10.1021/acs.nanolett.0c02576
State	Published - Oct 14 2020

Keywords

Diabetes
Glutamine
Islet transplantation
Mesoporous silica nanoparticles
Nutrient delivery
Polydopamine

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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@article{8d9c58da27934334aecf0fdb31df1906,

title = "Controlled Nutrient Delivery to Pancreatic Islets Using Polydopamine-Coated Mesoporous Silica Nanoparticles",

abstract = "In the present study, we created a nanoscale platform that can deliver nutrients to pancreatic islets in a controlled manner. Our platform consists of a mesoporous silica nanoparticle (MSNP), which can be loaded with glutamine (G: an essential amino acid required for islet survival and function). To control the release of G, MSNPs were coated with a polydopamine (PD) layer. With the optimal parameters (0.5 mg/mL and 0.5 h), MSNPs were coated with a layer of PD, which resulted in a delay of G release from MSNPs over 14 d (57.4 ± 4.7% release). Following syngeneic renal subcapsule islet transplantation in diabetic mice, PDG-MSNPs improved the engraftment of islets (i.e., enhanced revascularization and reduced inflammation) as well as their function, resulting in re-establishment of glycemic control. Collectively, our data show that PDG-MSNPs can support transplanted islets by providing them with a controlled and sustained supply of nutrients.",

keywords = "Diabetes, Glutamine, Islet transplantation, Mesoporous silica nanoparticles, Nutrient delivery, Polydopamine",

author = "Mehdi Razavi and Rosita Primavera and Kevadiya, {Bhavesh D.} and Jing Wang and Mujib Ullah and Peter Buchwald and Thakor, {Avnesh S.}",

note = "Funding Information: This work was supported by research grants from the NIDDK (R01DK119293 and P30DK116074), the Stanford Nano Shared Facilities grant (1161726-146-DAARZ), as part of the grant supported by the National Science Foundation grant (ECCS-1542152) and Stanford Neuroscience Microscopy Service grant (NIH NS069375) as well as the Akiko Yamazaki and Jerry Yang Faculty Scholar Fund in Pediatric Translational Medicine, and the Stanford Maternal and Child Health Research Institute. The authors thank Research Professor Y. J. Zhou, PhD. Director of Morrison Microscopy Core Research Facility, Center for Biotechnology, University of Nebraska-Lincoln for the technical support related to the TEM data. The authors also acknowledge A. Thomas for her help on preparing the TOC graphic.",

year = "2020",

month = oct,

day = "14",

doi = "10.1021/acs.nanolett.0c02576",

language = "English (US)",

volume = "20",

pages = "7220--7229",

journal = "Nano Letters",

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T1 - Controlled Nutrient Delivery to Pancreatic Islets Using Polydopamine-Coated Mesoporous Silica Nanoparticles

AU - Razavi, Mehdi

AU - Primavera, Rosita

AU - Kevadiya, Bhavesh D.

AU - Wang, Jing

AU - Ullah, Mujib

AU - Buchwald, Peter

AU - Thakor, Avnesh S.

N1 - Funding Information: This work was supported by research grants from the NIDDK (R01DK119293 and P30DK116074), the Stanford Nano Shared Facilities grant (1161726-146-DAARZ), as part of the grant supported by the National Science Foundation grant (ECCS-1542152) and Stanford Neuroscience Microscopy Service grant (NIH NS069375) as well as the Akiko Yamazaki and Jerry Yang Faculty Scholar Fund in Pediatric Translational Medicine, and the Stanford Maternal and Child Health Research Institute. The authors thank Research Professor Y. J. Zhou, PhD. Director of Morrison Microscopy Core Research Facility, Center for Biotechnology, University of Nebraska-Lincoln for the technical support related to the TEM data. The authors also acknowledge A. Thomas for her help on preparing the TOC graphic.

PY - 2020/10/14

Y1 - 2020/10/14

N2 - In the present study, we created a nanoscale platform that can deliver nutrients to pancreatic islets in a controlled manner. Our platform consists of a mesoporous silica nanoparticle (MSNP), which can be loaded with glutamine (G: an essential amino acid required for islet survival and function). To control the release of G, MSNPs were coated with a polydopamine (PD) layer. With the optimal parameters (0.5 mg/mL and 0.5 h), MSNPs were coated with a layer of PD, which resulted in a delay of G release from MSNPs over 14 d (57.4 ± 4.7% release). Following syngeneic renal subcapsule islet transplantation in diabetic mice, PDG-MSNPs improved the engraftment of islets (i.e., enhanced revascularization and reduced inflammation) as well as their function, resulting in re-establishment of glycemic control. Collectively, our data show that PDG-MSNPs can support transplanted islets by providing them with a controlled and sustained supply of nutrients.

AB - In the present study, we created a nanoscale platform that can deliver nutrients to pancreatic islets in a controlled manner. Our platform consists of a mesoporous silica nanoparticle (MSNP), which can be loaded with glutamine (G: an essential amino acid required for islet survival and function). To control the release of G, MSNPs were coated with a polydopamine (PD) layer. With the optimal parameters (0.5 mg/mL and 0.5 h), MSNPs were coated with a layer of PD, which resulted in a delay of G release from MSNPs over 14 d (57.4 ± 4.7% release). Following syngeneic renal subcapsule islet transplantation in diabetic mice, PDG-MSNPs improved the engraftment of islets (i.e., enhanced revascularization and reduced inflammation) as well as their function, resulting in re-establishment of glycemic control. Collectively, our data show that PDG-MSNPs can support transplanted islets by providing them with a controlled and sustained supply of nutrients.

KW - Diabetes

KW - Glutamine

KW - Islet transplantation

KW - Mesoporous silica nanoparticles

KW - Nutrient delivery

KW - Polydopamine

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JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

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