Controlled fabrication of a biological vascular substitute

Joel Stitzel, Jie Liu, Sang Jin Lee, Makoto Komura, Joel Berry, Shay Soker, Grace Lim, Mark Van Dyke, Richard Czerw, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticle

355 Citations (Scopus)

Abstract

Autologous and synthetic vessel grafts have been used as a vascular substitute for cardiovascular bypass procedures. However, these materials are limited by the availability of appropriate caliber autologous vessels, increased susceptibility to thrombosis and intimal hyperplasia following surgery. Electrospinning technology offers the potential for controlling composition, structure and mechanical properties of biomaterials. Vascular graft scaffolds have been fabricated using electrospun polymer blends of Type I collagen, elastin from ligamentum nuchae, and poly (d,l-lactide-co-glycolide). This study demonstrates improved electrospinning characteristics versus previous studies by increasing polymer concentration and adding PLGA to the polymer blend. Additionally, new in vitro biocompatibility and mechanical testing data is presented. The scaffolds possess tissue composition and mechanical properties similar to native vessels. The electrospun vessel matrix is biocompatible and does not elicit local or systemic toxic effects when implanted in vivo. This study demonstrates the promise of electrospinning as a fabrication process for a functional vascular graft for clinical use.

Original languageEnglish
Pages (from-to)1088-1094
Number of pages7
JournalBiomaterials
Volume27
Issue number7
DOIs
StatePublished - Mar 1 2006
Externally publishedYes

Fingerprint

Electrospinning
Grafts
Blood Vessels
Polymers
Polymer blends
Transplants
Fabrication
Tunica Intima
Tissue Scaffolds
Elastin
Materials Testing
Mechanical properties
Mechanical testing
Poisons
Biocompatible Materials
Collagen Type I
Chemical analysis
Biocompatibility
Collagen
Biomaterials

Keywords

  • Collagen
  • Elastin
  • Electrospinning
  • Mechanical properties
  • Scaffold
  • Vascular grafts

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Stitzel, J., Liu, J., Lee, S. J., Komura, M., Berry, J., Soker, S., ... Atala, A. (2006). Controlled fabrication of a biological vascular substitute. Biomaterials, 27(7), 1088-1094. https://doi.org/10.1016/j.biomaterials.2005.07.048

Controlled fabrication of a biological vascular substitute. / Stitzel, Joel; Liu, Jie; Lee, Sang Jin; Komura, Makoto; Berry, Joel; Soker, Shay; Lim, Grace; Van Dyke, Mark; Czerw, Richard; Yoo, James J.; Atala, Anthony.

In: Biomaterials, Vol. 27, No. 7, 01.03.2006, p. 1088-1094.

Research output: Contribution to journalArticle

Stitzel, J, Liu, J, Lee, SJ, Komura, M, Berry, J, Soker, S, Lim, G, Van Dyke, M, Czerw, R, Yoo, JJ & Atala, A 2006, 'Controlled fabrication of a biological vascular substitute', Biomaterials, vol. 27, no. 7, pp. 1088-1094. https://doi.org/10.1016/j.biomaterials.2005.07.048
Stitzel J, Liu J, Lee SJ, Komura M, Berry J, Soker S et al. Controlled fabrication of a biological vascular substitute. Biomaterials. 2006 Mar 1;27(7):1088-1094. https://doi.org/10.1016/j.biomaterials.2005.07.048
Stitzel, Joel ; Liu, Jie ; Lee, Sang Jin ; Komura, Makoto ; Berry, Joel ; Soker, Shay ; Lim, Grace ; Van Dyke, Mark ; Czerw, Richard ; Yoo, James J. ; Atala, Anthony. / Controlled fabrication of a biological vascular substitute. In: Biomaterials. 2006 ; Vol. 27, No. 7. pp. 1088-1094.
@article{adfb6071737d4911ab79c0f7b84ec5e9,
title = "Controlled fabrication of a biological vascular substitute",
abstract = "Autologous and synthetic vessel grafts have been used as a vascular substitute for cardiovascular bypass procedures. However, these materials are limited by the availability of appropriate caliber autologous vessels, increased susceptibility to thrombosis and intimal hyperplasia following surgery. Electrospinning technology offers the potential for controlling composition, structure and mechanical properties of biomaterials. Vascular graft scaffolds have been fabricated using electrospun polymer blends of Type I collagen, elastin from ligamentum nuchae, and poly (d,l-lactide-co-glycolide). This study demonstrates improved electrospinning characteristics versus previous studies by increasing polymer concentration and adding PLGA to the polymer blend. Additionally, new in vitro biocompatibility and mechanical testing data is presented. The scaffolds possess tissue composition and mechanical properties similar to native vessels. The electrospun vessel matrix is biocompatible and does not elicit local or systemic toxic effects when implanted in vivo. This study demonstrates the promise of electrospinning as a fabrication process for a functional vascular graft for clinical use.",
keywords = "Collagen, Elastin, Electrospinning, Mechanical properties, Scaffold, Vascular grafts",
author = "Joel Stitzel and Jie Liu and Lee, {Sang Jin} and Makoto Komura and Joel Berry and Shay Soker and Grace Lim and {Van Dyke}, Mark and Richard Czerw and Yoo, {James J.} and Anthony Atala",
year = "2006",
month = "3",
day = "1",
doi = "10.1016/j.biomaterials.2005.07.048",
language = "English",
volume = "27",
pages = "1088--1094",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "7",

}

TY - JOUR

T1 - Controlled fabrication of a biological vascular substitute

AU - Stitzel, Joel

AU - Liu, Jie

AU - Lee, Sang Jin

AU - Komura, Makoto

AU - Berry, Joel

AU - Soker, Shay

AU - Lim, Grace

AU - Van Dyke, Mark

AU - Czerw, Richard

AU - Yoo, James J.

AU - Atala, Anthony

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Autologous and synthetic vessel grafts have been used as a vascular substitute for cardiovascular bypass procedures. However, these materials are limited by the availability of appropriate caliber autologous vessels, increased susceptibility to thrombosis and intimal hyperplasia following surgery. Electrospinning technology offers the potential for controlling composition, structure and mechanical properties of biomaterials. Vascular graft scaffolds have been fabricated using electrospun polymer blends of Type I collagen, elastin from ligamentum nuchae, and poly (d,l-lactide-co-glycolide). This study demonstrates improved electrospinning characteristics versus previous studies by increasing polymer concentration and adding PLGA to the polymer blend. Additionally, new in vitro biocompatibility and mechanical testing data is presented. The scaffolds possess tissue composition and mechanical properties similar to native vessels. The electrospun vessel matrix is biocompatible and does not elicit local or systemic toxic effects when implanted in vivo. This study demonstrates the promise of electrospinning as a fabrication process for a functional vascular graft for clinical use.

AB - Autologous and synthetic vessel grafts have been used as a vascular substitute for cardiovascular bypass procedures. However, these materials are limited by the availability of appropriate caliber autologous vessels, increased susceptibility to thrombosis and intimal hyperplasia following surgery. Electrospinning technology offers the potential for controlling composition, structure and mechanical properties of biomaterials. Vascular graft scaffolds have been fabricated using electrospun polymer blends of Type I collagen, elastin from ligamentum nuchae, and poly (d,l-lactide-co-glycolide). This study demonstrates improved electrospinning characteristics versus previous studies by increasing polymer concentration and adding PLGA to the polymer blend. Additionally, new in vitro biocompatibility and mechanical testing data is presented. The scaffolds possess tissue composition and mechanical properties similar to native vessels. The electrospun vessel matrix is biocompatible and does not elicit local or systemic toxic effects when implanted in vivo. This study demonstrates the promise of electrospinning as a fabrication process for a functional vascular graft for clinical use.

KW - Collagen

KW - Elastin

KW - Electrospinning

KW - Mechanical properties

KW - Scaffold

KW - Vascular grafts

UR - http://www.scopus.com/inward/record.url?scp=27644501985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27644501985&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2005.07.048

DO - 10.1016/j.biomaterials.2005.07.048

M3 - Article

C2 - 16131465

AN - SCOPUS:27644501985

VL - 27

SP - 1088

EP - 1094

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 7

ER -