Abstract
Tissue engineered scaffolds should actively participate not only in structural support but also in functional tissue regeneration. Thus, novel smart biomaterial scaffolds have been developed, which incorporate a variety of bioactive molecules to accelerate neo-tissue formation. The effective delivery of multiple bioactive molecules with distinct kinetics to target sites at an appropriate concentration and in a timely manner is desired to drive tissue development to completion. To achieve effective, controllable delivery of multiple factors, a dual protein delivery system has been developed by electrospinning poly(lactide-co-glycolide) (PLGA) with different hydrophilicities. Bovine serum albumin or myoglobin was incorporated into and released gradually from these electrospun fibrous PLGA scaffolds. All the scaffolds exhibited similar loading efficiencies of approximately 80% of the target proteins. The introduction of Pluronic F-127 (PF127) dramatically increased scaffold hydrophilicity, which affected the release kinetics of these proteins from the scaffolds. Furthermore, distinct protein release patterns were achieved when using dual protein-loaded scaffolds with different hydrophilicities when these scaffolds were fabricated by co-electrospinning. This system may be useful as a method for delivering multiple bioactive vehicles for tissue engineering applications.
| Original language | English |
|---|---|
| Article number | 014104 |
| Journal | Biomedical Materials (Bristol) |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2013 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Chemistry (miscellaneous)
- Biochemistry
- Mechanics of Materials
Cite this
Controllable dual protein delivery through electrospun fibrous scaffolds with different hydrophilicities. / Xu, Weijie; Atala, Anthony; Yoo, James J.; Lee, Sang Jin.
In: Biomedical Materials (Bristol), Vol. 8, No. 1, 014104, 01.02.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Controllable dual protein delivery through electrospun fibrous scaffolds with different hydrophilicities
AU - Xu, Weijie
AU - Atala, Anthony
AU - Yoo, James J.
AU - Lee, Sang Jin
PY - 2013/2/1
Y1 - 2013/2/1
N2 - Tissue engineered scaffolds should actively participate not only in structural support but also in functional tissue regeneration. Thus, novel smart biomaterial scaffolds have been developed, which incorporate a variety of bioactive molecules to accelerate neo-tissue formation. The effective delivery of multiple bioactive molecules with distinct kinetics to target sites at an appropriate concentration and in a timely manner is desired to drive tissue development to completion. To achieve effective, controllable delivery of multiple factors, a dual protein delivery system has been developed by electrospinning poly(lactide-co-glycolide) (PLGA) with different hydrophilicities. Bovine serum albumin or myoglobin was incorporated into and released gradually from these electrospun fibrous PLGA scaffolds. All the scaffolds exhibited similar loading efficiencies of approximately 80% of the target proteins. The introduction of Pluronic F-127 (PF127) dramatically increased scaffold hydrophilicity, which affected the release kinetics of these proteins from the scaffolds. Furthermore, distinct protein release patterns were achieved when using dual protein-loaded scaffolds with different hydrophilicities when these scaffolds were fabricated by co-electrospinning. This system may be useful as a method for delivering multiple bioactive vehicles for tissue engineering applications.
AB - Tissue engineered scaffolds should actively participate not only in structural support but also in functional tissue regeneration. Thus, novel smart biomaterial scaffolds have been developed, which incorporate a variety of bioactive molecules to accelerate neo-tissue formation. The effective delivery of multiple bioactive molecules with distinct kinetics to target sites at an appropriate concentration and in a timely manner is desired to drive tissue development to completion. To achieve effective, controllable delivery of multiple factors, a dual protein delivery system has been developed by electrospinning poly(lactide-co-glycolide) (PLGA) with different hydrophilicities. Bovine serum albumin or myoglobin was incorporated into and released gradually from these electrospun fibrous PLGA scaffolds. All the scaffolds exhibited similar loading efficiencies of approximately 80% of the target proteins. The introduction of Pluronic F-127 (PF127) dramatically increased scaffold hydrophilicity, which affected the release kinetics of these proteins from the scaffolds. Furthermore, distinct protein release patterns were achieved when using dual protein-loaded scaffolds with different hydrophilicities when these scaffolds were fabricated by co-electrospinning. This system may be useful as a method for delivering multiple bioactive vehicles for tissue engineering applications.
UR - http://www.scopus.com/inward/record.url?scp=84873102487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873102487&partnerID=8YFLogxK
U2 - 10.1088/1748-6041/8/1/014104
DO - 10.1088/1748-6041/8/1/014104
M3 - Article
C2 - 23353662
AN - SCOPUS:84873102487
VL - 8
JO - Biomedical Materials
JF - Biomedical Materials
SN - 1748-6041
IS - 1
M1 - 014104
ER -