Controllable dual protein delivery through electrospun fibrous scaffolds with different hydrophilicities

Weijie Xu, Anthony Atala, James J. Yoo, Sang Jin Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tissue engineered scaffolds should actively participate not only in structural support but also in functional tissue regeneration. Thus, novel smart biomaterial scaffolds have been developed, which incorporate a variety of bioactive molecules to accelerate neo-tissue formation. The effective delivery of multiple bioactive molecules with distinct kinetics to target sites at an appropriate concentration and in a timely manner is desired to drive tissue development to completion. To achieve effective, controllable delivery of multiple factors, a dual protein delivery system has been developed by electrospinning poly(lactide-co-glycolide) (PLGA) with different hydrophilicities. Bovine serum albumin or myoglobin was incorporated into and released gradually from these electrospun fibrous PLGA scaffolds. All the scaffolds exhibited similar loading efficiencies of approximately 80% of the target proteins. The introduction of Pluronic F-127 (PF127) dramatically increased scaffold hydrophilicity, which affected the release kinetics of these proteins from the scaffolds. Furthermore, distinct protein release patterns were achieved when using dual protein-loaded scaffolds with different hydrophilicities when these scaffolds were fabricated by co-electrospinning. This system may be useful as a method for delivering multiple bioactive vehicles for tissue engineering applications.

Original languageEnglish
Article number014104
JournalBiomedical Materials (Bristol)
Volume8
Issue number1
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Fingerprint

Hydrophilicity
Scaffolds (biology)
Hydrophobic and Hydrophilic Interactions
Scaffolds
Proteins
Electrospinning
Tissue
Tissue Scaffolds
Polyglactin 910
Poloxamer
Tissue regeneration
Molecules
Kinetics
Myoglobin
Biocompatible Materials
Tissue Engineering
Bovine Serum Albumin
Tissue engineering
Biomaterials
Regeneration

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry
  • Mechanics of Materials

Cite this

Controllable dual protein delivery through electrospun fibrous scaffolds with different hydrophilicities. / Xu, Weijie; Atala, Anthony; Yoo, James J.; Lee, Sang Jin.

In: Biomedical Materials (Bristol), Vol. 8, No. 1, 014104, 01.02.2013.

Research output: Contribution to journalArticle

Xu, Weijie ; Atala, Anthony ; Yoo, James J. ; Lee, Sang Jin. / Controllable dual protein delivery through electrospun fibrous scaffolds with different hydrophilicities. In: Biomedical Materials (Bristol). 2013 ; Vol. 8, No. 1.
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