Control of ventilation in the hypercapnic skate Raja ocellata: I. Blood and extradural fluid

M. S. Graham, J. D. Turner, C. M. Wood

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

In order to study the role of CO2 and acid-base status in contributing to ventilatory drive, skate were exposed to normoxic hypercapnia (PICO2 = 7.5 Torr) under conditions where the primary O2 drive a small Bohr effect. Red blood cell pHi was not preferentially regulated, and there was no evidence of RBC swelling or nucleoside triphosphate adjustment. Although there were no changes in arterial O2 levels during hypercapnia, ventilation immediately increased 2.7-fold through large changes in stroke volume and small changes in frequency, and declined only slightly through 24-48 h. PaCO2 equilibrated rapidly with PICO2, driving down arterial pHa, which was 65% corrected through HCO3- accumulation by 24 h. In contrast, the extradural fluid outside the brain equilibrated only very slowly, and was clearly not involved in the ventilatory stimulation. Increased ventilation during hypercapnia may be related to depressions in pHa.

Original languageEnglish
Pages (from-to)259-277
Number of pages19
JournalRespiration Physiology
Volume80
Issue number2-3
DOIs
StatePublished - Jan 1 1990

Fingerprint

Skates (Fish)
Hypercapnia
Ventilation
Nucleosides
Stroke Volume
Erythrocytes
Acids
Brain

Keywords

  • Animal, skate
  • Blood, O transport properties in skate
  • Bohr effect in skate
  • Cerebrospinal fluid, acid-base balance
  • Control of breathing, cerebrospinal fluid in skate, response to CO
  • Hypercapnia, ventilatory response in skate
  • pH, intracellular
  • Red cell, intracellular pH in skate
  • Ventilation, sensitivity to CO in skate

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Control of ventilation in the hypercapnic skate Raja ocellata : I. Blood and extradural fluid. / Graham, M. S.; Turner, J. D.; Wood, C. M.

In: Respiration Physiology, Vol. 80, No. 2-3, 01.01.1990, p. 259-277.

Research output: Contribution to journalArticle

Graham, M. S. ; Turner, J. D. ; Wood, C. M. / Control of ventilation in the hypercapnic skate Raja ocellata : I. Blood and extradural fluid. In: Respiration Physiology. 1990 ; Vol. 80, No. 2-3. pp. 259-277.
@article{b7a8945f3b1f48009a3cd90102d391a6,
title = "Control of ventilation in the hypercapnic skate Raja ocellata: I. Blood and extradural fluid",
abstract = "In order to study the role of CO2 and acid-base status in contributing to ventilatory drive, skate were exposed to normoxic hypercapnia (PICO2 = 7.5 Torr) under conditions where the primary O2 drive a small Bohr effect. Red blood cell pHi was not preferentially regulated, and there was no evidence of RBC swelling or nucleoside triphosphate adjustment. Although there were no changes in arterial O2 levels during hypercapnia, ventilation immediately increased 2.7-fold through large changes in stroke volume and small changes in frequency, and declined only slightly through 24-48 h. PaCO2 equilibrated rapidly with PICO2, driving down arterial pHa, which was 65{\%} corrected through HCO3- accumulation by 24 h. In contrast, the extradural fluid outside the brain equilibrated only very slowly, and was clearly not involved in the ventilatory stimulation. Increased ventilation during hypercapnia may be related to depressions in pHa.",
keywords = "Animal, skate, Blood, O transport properties in skate, Bohr effect in skate, Cerebrospinal fluid, acid-base balance, Control of breathing, cerebrospinal fluid in skate, response to CO, Hypercapnia, ventilatory response in skate, pH, intracellular, Red cell, intracellular pH in skate, Ventilation, sensitivity to CO in skate",
author = "Graham, {M. S.} and Turner, {J. D.} and Wood, {C. M.}",
year = "1990",
month = "1",
day = "1",
doi = "10.1016/0034-5687(90)90088-G",
language = "English",
volume = "80",
pages = "259--277",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Control of ventilation in the hypercapnic skate Raja ocellata

T2 - I. Blood and extradural fluid

AU - Graham, M. S.

AU - Turner, J. D.

AU - Wood, C. M.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - In order to study the role of CO2 and acid-base status in contributing to ventilatory drive, skate were exposed to normoxic hypercapnia (PICO2 = 7.5 Torr) under conditions where the primary O2 drive a small Bohr effect. Red blood cell pHi was not preferentially regulated, and there was no evidence of RBC swelling or nucleoside triphosphate adjustment. Although there were no changes in arterial O2 levels during hypercapnia, ventilation immediately increased 2.7-fold through large changes in stroke volume and small changes in frequency, and declined only slightly through 24-48 h. PaCO2 equilibrated rapidly with PICO2, driving down arterial pHa, which was 65% corrected through HCO3- accumulation by 24 h. In contrast, the extradural fluid outside the brain equilibrated only very slowly, and was clearly not involved in the ventilatory stimulation. Increased ventilation during hypercapnia may be related to depressions in pHa.

AB - In order to study the role of CO2 and acid-base status in contributing to ventilatory drive, skate were exposed to normoxic hypercapnia (PICO2 = 7.5 Torr) under conditions where the primary O2 drive a small Bohr effect. Red blood cell pHi was not preferentially regulated, and there was no evidence of RBC swelling or nucleoside triphosphate adjustment. Although there were no changes in arterial O2 levels during hypercapnia, ventilation immediately increased 2.7-fold through large changes in stroke volume and small changes in frequency, and declined only slightly through 24-48 h. PaCO2 equilibrated rapidly with PICO2, driving down arterial pHa, which was 65% corrected through HCO3- accumulation by 24 h. In contrast, the extradural fluid outside the brain equilibrated only very slowly, and was clearly not involved in the ventilatory stimulation. Increased ventilation during hypercapnia may be related to depressions in pHa.

KW - Animal, skate

KW - Blood, O transport properties in skate

KW - Bohr effect in skate

KW - Cerebrospinal fluid, acid-base balance

KW - Control of breathing, cerebrospinal fluid in skate, response to CO

KW - Hypercapnia, ventilatory response in skate

KW - pH, intracellular

KW - Red cell, intracellular pH in skate

KW - Ventilation, sensitivity to CO in skate

UR - http://www.scopus.com/inward/record.url?scp=0025362669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025362669&partnerID=8YFLogxK

U2 - 10.1016/0034-5687(90)90088-G

DO - 10.1016/0034-5687(90)90088-G

M3 - Article

C2 - 2120753

AN - SCOPUS:0025362669

VL - 80

SP - 259

EP - 277

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 2-3

ER -