Control of IgE responses. III. IL-6 and IFN-α are isotype-specific regulators of peak BPO-specific IgE antibody-forming cell responses in mice

Dominick L. Auci, Gary I. Kleiner, Seto M. Chice, Peter Dukor, Helen G. Durkin

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The ability of cytokines (IL-4, IL-5, IL-6, IFN-α, IFN-γ, TNF-α, GmCSF) to regulate peak benzylpenicilloyl (BPO)-specific IgE antibody-forming cell (AFC) responses was investigated. These responses were induced in BALB/c mice by ip injection of BPO-keyhole limpet hemocyanin (BPO-KLH; 10 μg) in aluminum hydroxide gel on Days 0, 21, and 42. On Day 44, or on Days 43, 44, and 45, mice were injected sc with varying doses of cytokine or anti-cytokine antibody. On Day 46, the numbers of BPO-specific AFC (IgM, IgG1, IgE and IgA) in spleen were determined ex vivo in enzyme-linked immunosorbent spot assay. Among the cytokines tested, only IL-6 suppressed BPO-specific IgE AFC responses in an isotype-specific fashion (60-90%). However, treatment of mice with anti-IL-6 also suppressed these responses, suggesting that IL-6 can either suppress or increase peak antigen specific IgE responses, depending upon its concentration. Among the cytokines tested, only IFN-α increased BPO-specific IgE AFC responses in an isotype-specific fashion. Since treatment with anti-IFN-α suppressed these responses, it appears that IFN-α is required to maintain peak antigen-specific IgE AFC responses. IL-4 or IFN-γ nonspecifically suppressed responses of all isotypes. Treatment with anti-IL-4 also suppressed IgE responses, suggesting that this cytokine is required to maintain peak antigen specific IgE responses. Treatment with anti-IFN-γ increased IgE responses, indicating that IFN-γ suppresses peak antigen-specific IgE responses.

Original languageEnglish (US)
Pages (from-to)219-224
Number of pages6
JournalClinical Immunology and Immunopathology
Volume66
Issue number3
DOIs
StatePublished - Mar 1993
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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