Contribution of Tamm-Horsfall protein to virulence of Pseudomonas aeruginosa in urinary tract infection

Kusum Harjai, Rahul Mittal, Sanjay Chhibber, Saroj Sharma

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Tamm-Horsfall glycoprotein (THP) is the most abundant protein which is synthesized by renal tubular cells and excreted in urine. Its role in urinary tract infection has yet not been identified. In the present study, the contribution of THP towards adherence of Pseudomonas aeruginosa to uroepithelial cells and murine peritoneal macrophages was studied. Decreased adherence of THP-coated P. aeruginosa to UECs and phagocytes was observed in vitro. In vivo, P. aeruginosa showed increased renal bacterial load and tissue pathology in a mouse model of acute ascending pyelonephritis, when THP-coated P. aeruginosa was used to cause infection. This study shows that THP may not necessarily act as a host defense component; rather, it may help in renal colonization of P. aeruginosa in vivo.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalMicrobes and Infection
Volume7
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Keywords

  • Macrophages
  • Pseudomonas aeruginosa
  • Tamm-Horsfall protein
  • Urinary tract infection
  • Uroepithelial cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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