Contribution of quorum-sensing systems to virulence of Pseudomonas aeruginosa in an experimental pyelonephritis model

Rahul Mittal, Saroj Sharma, Sanjay Chhibber, Kusum Harjai

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Background and Purpose: Pseudomonas aeruginosa has been reported to monitor its cell density as well as expression of virulence determinants by quorum-sensing signal mechanisms operative through autoinducers. In the present investigation, we studied the contribution of quorum-sensing signals during the course of P. aeruginosa-induced pyelonephritis in mice. Methods: The standard parent strain of P. aeruginosa (PAO1), possessing functional las and rhl quorum-sensing systems and its isogenic mutant strains, PAO-JP1 (single mutant), harboring a mutated /asl gene and PAO-JP3 (double mutant), harboring mutated lasI and rhlR genes were employed. One uroisolate of P. aeruginosa belonging to serotype O8 and deficient in production of quorum-sensing signals was also used. Results: The parent strain of P. aeruginosa was significantly more virulent compared to its isogenic mutant strains and quorum-sensing negative clinical strain, as assessed by neutrophil influx, malondialdehyde production, renal bacterial load and pathology induced in experimental animals. Conclusions: Quorum-sensing systems play an important role in the pathogenicity of P. aeruginosa in pyelonephritis. Both the las and rhl quorum-sensing systems are important for the virulence of P. aeruginosa in the development of pyelonephritis.

Original languageEnglish (US)
Pages (from-to)302-309
Number of pages8
JournalJournal of Microbiology, Immunology and Infection
Volume39
Issue number4
StatePublished - Aug 2006
Externally publishedYes

Keywords

  • Animal disease model
  • Pseudomonas aeruginosa
  • Quorum sensing
  • Virulence

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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