Contribution of lipoproteins and lipoprotein processing to endocarditis virulence in Streptococcus sanguinis

Sankar Das, Taisei Kanamoto, Xiuchun Ge, Ping Xu, Takeshi Unoki, Cindy Munro, Todd Kitten

Research output: Contribution to journalArticle

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Abstract

Streptococcus sanguinis is an important cause of infective endocarditis. Previous studies have identified lipoproteins as virulence determinants in other streptococcal species. Using a bioinformatic approach, we identified 52 putative lipoprotein genes in S. sanguinis strain SK36 as well as genes encoding the lipoprotein-processing enzymes prolipoprotein diacylglyceryl transferase (lgt) and signal peptidase II (lspA). We employed a directed signature-tagged mutagenesis approach to systematically disrupt these genes and screen each mutant for the loss of virulence in an animal model of endocarditis. All mutants were viable. In competitive index assays, mutation of a putative phosphate transporter reduced in vivo competitiveness by 14-fold but also reduced in vitro viability by more than 20-fold. Mutations in lgt, lspA, or an uncharacterized lipoprotein gene reduced competitiveness by two- to threefold in the animal model and in broth culture. Mutation of ssaB, encoding a putative metal transporter, produced a similar effect in culture but reduced in vivo competiveness by >1,000-fold. [3H]palmitate labeling and Western blot analysis confirmed that the lgt mutant failed to acylate lipoproteins, that the lspA mutant had a general defect in lipoprotein cleavage, and that SsaB was processed differently in both mutants. These results indicate that the loss of a single lipoprotein, SsaB, dramatically reduces endocarditis virulence, whereas the loss of most other lipoproteins or of normal lipoprotein processing has no more than a minor effect on virulence.

Original languageEnglish (US)
Pages (from-to)4166-4179
Number of pages14
JournalJournal of Bacteriology
Volume191
Issue number13
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

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Endocarditis
Streptococcus
Lipoproteins
Virulence
Mutation
Genes
Animal Models
Phosphate Transport Proteins
Palmitates
Computational Biology
Mutagenesis
Western Blotting
Metals
signal peptidase II
Enzymes
Streptococcus sanguis 12 saliva-binding protein

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Contribution of lipoproteins and lipoprotein processing to endocarditis virulence in Streptococcus sanguinis. / Das, Sankar; Kanamoto, Taisei; Ge, Xiuchun; Xu, Ping; Unoki, Takeshi; Munro, Cindy; Kitten, Todd.

In: Journal of Bacteriology, Vol. 191, No. 13, 01.07.2009, p. 4166-4179.

Research output: Contribution to journalArticle

Das, Sankar ; Kanamoto, Taisei ; Ge, Xiuchun ; Xu, Ping ; Unoki, Takeshi ; Munro, Cindy ; Kitten, Todd. / Contribution of lipoproteins and lipoprotein processing to endocarditis virulence in Streptococcus sanguinis. In: Journal of Bacteriology. 2009 ; Vol. 191, No. 13. pp. 4166-4179.
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