Contribution of calcium channel subtypes to the intracellular calcium signal in sensory neurons: The effect of injury

Andreas Fuchs, Marcel Rigaud, Konstantinos D. Sarantopoulos, Patrick Filip, Quinn H. Hogan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

BACKGROUND: Although the activation-induced intracellular Ca signal is disrupted by sensory neuron injury, the contribution of specific Ca channel subtypes is unknown. METHODS: Transients in dissociated rat dorsal root ganglion neurons were recorded using fura-2 microfluorometry. Neurons from control rats and from neuropathic animals after spinal nerve ligation were activated either by elevated bath K or by field stimulation. Transients were compared before and after application of selective blockers of voltage-activated Ca channel subtypes. RESULTS: Transient amplitude and area were decreased by blockade of the L-type channel, particularly during sustained K stimulation. Significant contributions to the Ca transient are attributable to the N-, P/Q-, and R-type channels, especially in small neurons. Results for T-type blockade varied widely between cells. After injury, transients lost sensitivity to N-type and R-type blockers in axotomized small neurons, whereas adjacent small neurons showed decreased responses to blockers of R-type channels. Axotomized large neurons were less sensitive to blockade of N- and P/Q-type channels. After injury, neurons adjacent to axotomy show decreased sensitivity of K-induced transients to L-type blockade but increased sensitivity during field stimulation. CONCLUSIONS: All high-voltage-activated Ca current subtypes contribute to Ca transients in sensory neurons, although the L-type channel contributes predominantly during prolonged activation. Injury shifts the relative contribution of various Ca channel subtypes to the intracellular Ca transient induced by neuronal activation. Because this effect is cell-size specific, selective therapies might potentially be devised to differentially alter excitability of nociceptive and low-threshold sensory neurons.

Original languageEnglish
Pages (from-to)117-127
Number of pages11
JournalAnesthesiology
Volume107
Issue number1
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

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Sensory Receptor Cells
Calcium Channels
Calcium
Neurons
Wounds and Injuries
Cytophotometry
Axotomy
Spinal Nerves
Fura-2
Spinal Ganglia
Baths
Cell Size
Ligation

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Contribution of calcium channel subtypes to the intracellular calcium signal in sensory neurons : The effect of injury. / Fuchs, Andreas; Rigaud, Marcel; Sarantopoulos, Konstantinos D.; Filip, Patrick; Hogan, Quinn H.

In: Anesthesiology, Vol. 107, No. 1, 01.07.2007, p. 117-127.

Research output: Contribution to journalArticle

Fuchs, Andreas ; Rigaud, Marcel ; Sarantopoulos, Konstantinos D. ; Filip, Patrick ; Hogan, Quinn H. / Contribution of calcium channel subtypes to the intracellular calcium signal in sensory neurons : The effect of injury. In: Anesthesiology. 2007 ; Vol. 107, No. 1. pp. 117-127.
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N2 - BACKGROUND: Although the activation-induced intracellular Ca signal is disrupted by sensory neuron injury, the contribution of specific Ca channel subtypes is unknown. METHODS: Transients in dissociated rat dorsal root ganglion neurons were recorded using fura-2 microfluorometry. Neurons from control rats and from neuropathic animals after spinal nerve ligation were activated either by elevated bath K or by field stimulation. Transients were compared before and after application of selective blockers of voltage-activated Ca channel subtypes. RESULTS: Transient amplitude and area were decreased by blockade of the L-type channel, particularly during sustained K stimulation. Significant contributions to the Ca transient are attributable to the N-, P/Q-, and R-type channels, especially in small neurons. Results for T-type blockade varied widely between cells. After injury, transients lost sensitivity to N-type and R-type blockers in axotomized small neurons, whereas adjacent small neurons showed decreased responses to blockers of R-type channels. Axotomized large neurons were less sensitive to blockade of N- and P/Q-type channels. After injury, neurons adjacent to axotomy show decreased sensitivity of K-induced transients to L-type blockade but increased sensitivity during field stimulation. CONCLUSIONS: All high-voltage-activated Ca current subtypes contribute to Ca transients in sensory neurons, although the L-type channel contributes predominantly during prolonged activation. Injury shifts the relative contribution of various Ca channel subtypes to the intracellular Ca transient induced by neuronal activation. Because this effect is cell-size specific, selective therapies might potentially be devised to differentially alter excitability of nociceptive and low-threshold sensory neurons.

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