Contractile responses of cultured bovine retinal pericytes to angiotensin II

Taheshi Matsugi, Qian Chcn, Douglas Anderson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective: To document that angiotensin (ANG) II contracts cultured bovine retinal pericytes via saralasin-sensitive receptors if the cells are prerelaxed. Methods: Changes in the contractile tone were quantified as the changes in the summed length of wrinkles induced by pericytes cultured on the silicone surface. Results: Angiotensin II (10-5 mol/L) did not increase the contractile tone of cultured pericytes that were not prerelaxed. However, when the pericytes had been prerelaxed 41% with 10-6-mol/L sodium nitroprusside, ANG II at the range of 10-7 to 10-5 mol/L caused prompt, dose related, significant (P<.01) contraction. It induced a maximum contraction (29.9%±5.2% [mean±SE]) at 10-6 mol/L. This effect lasted at least 10 minutes. Angiotensin II receptor antagonist saralasin (10-6 mol/L) abolished the contractile effect of ANG II (10-6 mol/L), although by itself it did not affect the contractile tone. Conclusions: Angiotensin II contracts cultured pericytes through saralasin-sensitive ANG II receptors. If ANG II affects the contractile tone of pericytes in vivo, it may affect capillary caliber, resistance, and blood flow.

Original languageEnglish
Pages (from-to)1281-1285
Number of pages5
JournalArchives of Ophthalmology
Volume115
Issue number10
StatePublished - Oct 22 1997

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Pericytes
Angiotensin II
Saralasin
Contracts
Capillary Resistance
Angiotensin Receptors
Angiotensin Receptor Antagonists
Nitroprusside
Silicones

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Matsugi, T., Chcn, Q., & Anderson, D. (1997). Contractile responses of cultured bovine retinal pericytes to angiotensin II. Archives of Ophthalmology, 115(10), 1281-1285.

Contractile responses of cultured bovine retinal pericytes to angiotensin II. / Matsugi, Taheshi; Chcn, Qian; Anderson, Douglas.

In: Archives of Ophthalmology, Vol. 115, No. 10, 22.10.1997, p. 1281-1285.

Research output: Contribution to journalArticle

Matsugi, T, Chcn, Q & Anderson, D 1997, 'Contractile responses of cultured bovine retinal pericytes to angiotensin II', Archives of Ophthalmology, vol. 115, no. 10, pp. 1281-1285.
Matsugi, Taheshi ; Chcn, Qian ; Anderson, Douglas. / Contractile responses of cultured bovine retinal pericytes to angiotensin II. In: Archives of Ophthalmology. 1997 ; Vol. 115, No. 10. pp. 1281-1285.
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