Objective: To document that angiotensin (ANG) II contracts cultured bovine retinal pericytes via saralasin-sensitive receptors if the cells are prerelaxed. Methods: Changes in the contractile tone were quantified as the changes in the summed length of wrinkles induced by pericytes cultured on the silicone surface. Results: Angiotensin II (10-5 mol/L) did not increase the contractile tone of cultured pericytes that were not prerelaxed. However, when the pericytes had been prerelaxed 41% with 10-6-mol/L sodium nitroprusside, ANG II at the range of 10-7 to 10-5 mol/L caused prompt, dose related, significant (P<.01) contraction. It induced a maximum contraction (29.9%±5.2% [mean±SE]) at 10-6 mol/L. This effect lasted at least 10 minutes. Angiotensin II receptor antagonist saralasin (10-6 mol/L) abolished the contractile effect of ANG II (10-6 mol/L), although by itself it did not affect the contractile tone. Conclusions: Angiotensin II contracts cultured pericytes through saralasin-sensitive ANG II receptors. If ANG II affects the contractile tone of pericytes in vivo, it may affect capillary caliber, resistance, and blood flow.
|Original language||English (US)|
|Number of pages||5|
|Journal||Archives of ophthalmology|
|State||Published - 1997|
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