Continuous expression of corticotropin-releasing factor in the central nucleus of the amygdala emulates the dysregulation of the stress and reproductive axes

E. Keen-Rhinehart, V. Michopoulos, D. J. Toufexis, E. I. Martin, H. Nair, K. J. Ressler, M. Davis, M. J. Owens, Charles Nemeroff, M. E. Wilson

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

An increase in corticotropin-releasing factor (CRF) is a putative factor in the pathophysiology of stress-related disorders. As CRF expression in the central nucleus of the amygdala (CeA) is important in adaptation to chronic stress, we hypothesized that unrestrained synthesis of CRF in CeA would mimic the consequences of chronic stress exposure and cause dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis, increase emotionality and disrupt reproduction. To test this hypothesis, we used a lentiviral vector to increase CRF-expression site specifically in CeA of female rats. Increased synthesis of CRF in CeA amplified CRF and arginine vasopressin peptide concentration in the paraventricular nucleus of the hypothalamus, and decreased glucocorticoid negative feedback, both markers associated with the pathophysiology of depression. In addition, continuous expression of CRF in CeA also increased the acoustic startle response and depressive-like behavior in the forced swim test. Protein levels of gonadotropin-releasing hormone in the medial preoptic area were significantly reduced by continuous expression of CRF in CeA and this was associated with a lengthening of estrous cycles. Finally, sexual motivation but not sexual receptivity was significantly attenuated by continuous CRF synthesis in ovariectomized estradiol-progesterone-primed females. These data indicate that unrestrained CRF synthesis in CeA produces a dysregulation of the HPA axis, as well as many of the behavioral, physiological and reproductive consequences associated with stress-related disorders.

Original languageEnglish
Pages (from-to)37-50
Number of pages14
JournalMolecular Psychiatry
Volume14
Issue number1
DOIs
StatePublished - Jan 25 2009
Externally publishedYes

Fingerprint

Corticotropin-Releasing Hormone
Startle Reflex
Central Amygdaloid Nucleus
Preoptic Area
Estrous Cycle
Arginine Vasopressin
Paraventricular Hypothalamic Nucleus
Acoustics
Gonadotropin-Releasing Hormone
Glucocorticoids
Hypothalamus
Reproduction
Progesterone
Motivation
Estradiol
Depression
Peptides

Keywords

  • CeA
  • CRF
  • Emotionality
  • Fertility
  • Sexual motivation
  • Stress

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

Cite this

Continuous expression of corticotropin-releasing factor in the central nucleus of the amygdala emulates the dysregulation of the stress and reproductive axes. / Keen-Rhinehart, E.; Michopoulos, V.; Toufexis, D. J.; Martin, E. I.; Nair, H.; Ressler, K. J.; Davis, M.; Owens, M. J.; Nemeroff, Charles; Wilson, M. E.

In: Molecular Psychiatry, Vol. 14, No. 1, 25.01.2009, p. 37-50.

Research output: Contribution to journalArticle

Keen-Rhinehart, E, Michopoulos, V, Toufexis, DJ, Martin, EI, Nair, H, Ressler, KJ, Davis, M, Owens, MJ, Nemeroff, C & Wilson, ME 2009, 'Continuous expression of corticotropin-releasing factor in the central nucleus of the amygdala emulates the dysregulation of the stress and reproductive axes', Molecular Psychiatry, vol. 14, no. 1, pp. 37-50. https://doi.org/10.1038/mp.2008.91
Keen-Rhinehart, E. ; Michopoulos, V. ; Toufexis, D. J. ; Martin, E. I. ; Nair, H. ; Ressler, K. J. ; Davis, M. ; Owens, M. J. ; Nemeroff, Charles ; Wilson, M. E. / Continuous expression of corticotropin-releasing factor in the central nucleus of the amygdala emulates the dysregulation of the stress and reproductive axes. In: Molecular Psychiatry. 2009 ; Vol. 14, No. 1. pp. 37-50.
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