Continuous exposure to bright light upregulates bFGF and CNTF expression in the rat retina

Rong Wen, Tong Cheng, Ying Song, Michael T. Matthes, Douglas Yasumura, Matthew M. LaVail, Roy H. Steinberg

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Purpose. To examine mRNA expression of neurotrophic factors in the retina after exposure to bright light. Methods. Male adult Sprague-Dawley rats were exposed to light of 115-130 ft-c. Retinas were collected after 1, 2, 4 or 7 days of exposure. Northern blot analysis was performed to determine mRNA levels for the following factors and their receptors: basic fibroblast growth factor (bFGF), ciliary neurotrophic factor (CNTF), acidic fibroblast growth factor (aFGF), brain-derived neurotrophic factor (BDNF), insulin-like growth factor 1 (IGF-1) and glial fibrillary acidic protein (GFAP). Expression of bFGE CNTF and GFAP was localized by in situ hybridization. Results. Exposure to light of 115-130 ft-c resulted in a substantial increase in bFGF and CNTF expression that persisted during the entire 7-day period of exposure. The peak expression of bFGF was almost 9-fold at day 2. The increase in CNTF mRNA reached a maximum of 6-fold at day 4. A small increase (50%) in IGF-1 mRNA was also seen at day 4. Among the receptors, an elevation of 3-fold in FGF receptor 1 (FGFR-1) was observed at day 2. There was also a small increase (70%) in IGF-1 receptor (IGF-1R) at day 2. In addition, the expression of GFAP showed a rapid elevation of about 8-fold by day 1 and 9-fold by day 2, and 18-fold by day it. There was, however, no significant alteration in the expression of aFGF and BDNE. In situ hybridizations showed that the elevation of bFGF, CNTF and GFAP occurred across the entire retina with especially prominent increases over specific layers for each gene. Conclusions. Continuous exposure to bright light upregulates bFGF, CNTF, FGFR-1 and GFAP expression in the rat retina. The pattern of induced expression closely resembles that induced by mechanical injury, implying a common underlying mechanism.

Original languageEnglish
Pages (from-to)494-500
Number of pages7
JournalCurrent Eye Research
Volume17
Issue number5
DOIs
StatePublished - Jul 15 1998
Externally publishedYes

Fingerprint

Ciliary Neurotrophic Factor
Glial Fibrillary Acidic Protein
Fibroblast Growth Factor 2
Fibroblast Growth Factor 1
Retina
Up-Regulation
Light
Fibroblast Growth Factor Receptors
Messenger RNA
Somatomedins
In Situ Hybridization
Ciliary Neurotrophic Factor Receptor
Somatomedin Receptors
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Northern Blotting
Sprague Dawley Rats
Wounds and Injuries
Genes

Keywords

  • Light damage
  • Muller cells
  • Neurotrophic factors
  • Photoreceptors
  • Retinal injury

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Wen, R., Cheng, T., Song, Y., Matthes, M. T., Yasumura, D., LaVail, M. M., & Steinberg, R. H. (1998). Continuous exposure to bright light upregulates bFGF and CNTF expression in the rat retina. Current Eye Research, 17(5), 494-500. https://doi.org/10.1076/ceyr.17.5.494.5186

Continuous exposure to bright light upregulates bFGF and CNTF expression in the rat retina. / Wen, Rong; Cheng, Tong; Song, Ying; Matthes, Michael T.; Yasumura, Douglas; LaVail, Matthew M.; Steinberg, Roy H.

In: Current Eye Research, Vol. 17, No. 5, 15.07.1998, p. 494-500.

Research output: Contribution to journalArticle

Wen, R, Cheng, T, Song, Y, Matthes, MT, Yasumura, D, LaVail, MM & Steinberg, RH 1998, 'Continuous exposure to bright light upregulates bFGF and CNTF expression in the rat retina', Current Eye Research, vol. 17, no. 5, pp. 494-500. https://doi.org/10.1076/ceyr.17.5.494.5186
Wen, Rong ; Cheng, Tong ; Song, Ying ; Matthes, Michael T. ; Yasumura, Douglas ; LaVail, Matthew M. ; Steinberg, Roy H. / Continuous exposure to bright light upregulates bFGF and CNTF expression in the rat retina. In: Current Eye Research. 1998 ; Vol. 17, No. 5. pp. 494-500.
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AU - Wen, Rong

AU - Cheng, Tong

AU - Song, Ying

AU - Matthes, Michael T.

AU - Yasumura, Douglas

AU - LaVail, Matthew M.

AU - Steinberg, Roy H.

PY - 1998/7/15

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N2 - Purpose. To examine mRNA expression of neurotrophic factors in the retina after exposure to bright light. Methods. Male adult Sprague-Dawley rats were exposed to light of 115-130 ft-c. Retinas were collected after 1, 2, 4 or 7 days of exposure. Northern blot analysis was performed to determine mRNA levels for the following factors and their receptors: basic fibroblast growth factor (bFGF), ciliary neurotrophic factor (CNTF), acidic fibroblast growth factor (aFGF), brain-derived neurotrophic factor (BDNF), insulin-like growth factor 1 (IGF-1) and glial fibrillary acidic protein (GFAP). Expression of bFGE CNTF and GFAP was localized by in situ hybridization. Results. Exposure to light of 115-130 ft-c resulted in a substantial increase in bFGF and CNTF expression that persisted during the entire 7-day period of exposure. The peak expression of bFGF was almost 9-fold at day 2. The increase in CNTF mRNA reached a maximum of 6-fold at day 4. A small increase (50%) in IGF-1 mRNA was also seen at day 4. Among the receptors, an elevation of 3-fold in FGF receptor 1 (FGFR-1) was observed at day 2. There was also a small increase (70%) in IGF-1 receptor (IGF-1R) at day 2. In addition, the expression of GFAP showed a rapid elevation of about 8-fold by day 1 and 9-fold by day 2, and 18-fold by day it. There was, however, no significant alteration in the expression of aFGF and BDNE. In situ hybridizations showed that the elevation of bFGF, CNTF and GFAP occurred across the entire retina with especially prominent increases over specific layers for each gene. Conclusions. Continuous exposure to bright light upregulates bFGF, CNTF, FGFR-1 and GFAP expression in the rat retina. The pattern of induced expression closely resembles that induced by mechanical injury, implying a common underlying mechanism.

AB - Purpose. To examine mRNA expression of neurotrophic factors in the retina after exposure to bright light. Methods. Male adult Sprague-Dawley rats were exposed to light of 115-130 ft-c. Retinas were collected after 1, 2, 4 or 7 days of exposure. Northern blot analysis was performed to determine mRNA levels for the following factors and their receptors: basic fibroblast growth factor (bFGF), ciliary neurotrophic factor (CNTF), acidic fibroblast growth factor (aFGF), brain-derived neurotrophic factor (BDNF), insulin-like growth factor 1 (IGF-1) and glial fibrillary acidic protein (GFAP). Expression of bFGE CNTF and GFAP was localized by in situ hybridization. Results. Exposure to light of 115-130 ft-c resulted in a substantial increase in bFGF and CNTF expression that persisted during the entire 7-day period of exposure. The peak expression of bFGF was almost 9-fold at day 2. The increase in CNTF mRNA reached a maximum of 6-fold at day 4. A small increase (50%) in IGF-1 mRNA was also seen at day 4. Among the receptors, an elevation of 3-fold in FGF receptor 1 (FGFR-1) was observed at day 2. There was also a small increase (70%) in IGF-1 receptor (IGF-1R) at day 2. In addition, the expression of GFAP showed a rapid elevation of about 8-fold by day 1 and 9-fold by day 2, and 18-fold by day it. There was, however, no significant alteration in the expression of aFGF and BDNE. In situ hybridizations showed that the elevation of bFGF, CNTF and GFAP occurred across the entire retina with especially prominent increases over specific layers for each gene. Conclusions. Continuous exposure to bright light upregulates bFGF, CNTF, FGFR-1 and GFAP expression in the rat retina. The pattern of induced expression closely resembles that induced by mechanical injury, implying a common underlying mechanism.

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KW - Muller cells

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KW - Photoreceptors

KW - Retinal injury

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