Construction of cDNA expression library from the caudal spine of Dasyatis akajei

Hong Bin Tu; Jian Wen Wei; Xiao Yu Jiang; Hui Ping Chen; Xiao Fen Zhong; Wen Yan Wu; An Long Xu

Construction of cDNA expression library from the caudal spine of Dasyatis akajei

Hong Bin Tu, Jian Wen Wei, Xiao Yu Jiang, Hui Ping Chen, Xiao Fen Zhong, Wen Yan Wu, An Long Xu

Research output: Contribution to journal › Article › peer-review

Abstract

AIM A cDNA expressional library from the caudal spine of Dasyatis akajei was constructed into pcDNA 3.0 eukaryotical expression plasmid. METHODS SMART™ protocol was used for cDNA Library Construction and bioinformatics analysis was carried out. RESULTS Fifty six novel EST clones were obtained from the library, in which there were 24 full-length clones, including IPL tumor suppressor gene, cyclophilin A, selenoprotein W, cystatin A, tryosyl-tRNA synthetase and calcineurin homologous protein. CONCLUSION These genes were mostly first reported in Dasyatis akajei. Further studies may partly explain the molecular mechanisms by which the caudal spine of Dasyatis akajei were often used in the treatment of inflammation, parasitosis and cancer in the traditional Chinese medicine.

Original language	English (US)
Number of pages	1
Journal	Yaoxue Xuebao
Volume	36
Issue number	12
State	Published - Dec 1 2001
Externally published	Yes

Keywords

Caudal spine
Dasyatis akajei
cDNA expression library

ASJC Scopus subject areas

Molecular Medicine
Pharmacology, Toxicology and Pharmaceutics(all)

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@article{98772f1919ce46c0b82e206e94f7b6e0,

title = "Construction of cDNA expression library from the caudal spine of Dasyatis akajei",

abstract = "AIM A cDNA expressional library from the caudal spine of Dasyatis akajei was constructed into pcDNA 3.0 eukaryotical expression plasmid. METHODS SMART{\texttrademark} protocol was used for cDNA Library Construction and bioinformatics analysis was carried out. RESULTS Fifty six novel EST clones were obtained from the library, in which there were 24 full-length clones, including IPL tumor suppressor gene, cyclophilin A, selenoprotein W, cystatin A, tryosyl-tRNA synthetase and calcineurin homologous protein. CONCLUSION These genes were mostly first reported in Dasyatis akajei. Further studies may partly explain the molecular mechanisms by which the caudal spine of Dasyatis akajei were often used in the treatment of inflammation, parasitosis and cancer in the traditional Chinese medicine.",

keywords = "Caudal spine, Dasyatis akajei, cDNA expression library",

author = "Tu, {Hong Bin} and Wei, {Jian Wen} and Jiang, {Xiao Yu} and Chen, {Hui Ping} and Zhong, {Xiao Fen} and Wu, {Wen Yan} and Xu, {An Long}",

year = "2001",

month = dec,

day = "1",

language = "English (US)",

volume = "36",

journal = "Yao xue xue bao = Acta pharmaceutica Sinica",

issn = "0513-4870",

publisher = "Zhonghua Yixuehui Zazhishe",

number = "12",

}

TY - JOUR

T1 - Construction of cDNA expression library from the caudal spine of Dasyatis akajei

AU - Tu, Hong Bin

AU - Wei, Jian Wen

AU - Jiang, Xiao Yu

AU - Chen, Hui Ping

AU - Zhong, Xiao Fen

AU - Wu, Wen Yan

AU - Xu, An Long

PY - 2001/12/1

Y1 - 2001/12/1

N2 - AIM A cDNA expressional library from the caudal spine of Dasyatis akajei was constructed into pcDNA 3.0 eukaryotical expression plasmid. METHODS SMART™ protocol was used for cDNA Library Construction and bioinformatics analysis was carried out. RESULTS Fifty six novel EST clones were obtained from the library, in which there were 24 full-length clones, including IPL tumor suppressor gene, cyclophilin A, selenoprotein W, cystatin A, tryosyl-tRNA synthetase and calcineurin homologous protein. CONCLUSION These genes were mostly first reported in Dasyatis akajei. Further studies may partly explain the molecular mechanisms by which the caudal spine of Dasyatis akajei were often used in the treatment of inflammation, parasitosis and cancer in the traditional Chinese medicine.

AB - AIM A cDNA expressional library from the caudal spine of Dasyatis akajei was constructed into pcDNA 3.0 eukaryotical expression plasmid. METHODS SMART™ protocol was used for cDNA Library Construction and bioinformatics analysis was carried out. RESULTS Fifty six novel EST clones were obtained from the library, in which there were 24 full-length clones, including IPL tumor suppressor gene, cyclophilin A, selenoprotein W, cystatin A, tryosyl-tRNA synthetase and calcineurin homologous protein. CONCLUSION These genes were mostly first reported in Dasyatis akajei. Further studies may partly explain the molecular mechanisms by which the caudal spine of Dasyatis akajei were often used in the treatment of inflammation, parasitosis and cancer in the traditional Chinese medicine.

KW - Caudal spine

KW - Dasyatis akajei

KW - cDNA expression library

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