Construction of cDNA expression library from the caudal spine of Dasyatis akajei

Hong Bin Tu, Jian Wen Wei, Xiao Yu Jiang, Hui Ping Chen, Xiao Fen Zhong, Wen Yan Wu, An Long Xu

Research output: Contribution to journal › Article

Abstract

AIM A cDNA expressional library from the caudal spine of Dasyatis akajei was constructed into pcDNA 3.0 eukaryotical expression plasmid. METHODS SMART™ protocol was used for cDNA Library Construction and bioinformatics analysis was carried out. RESULTS Fifty six novel EST clones were obtained from the library, in which there were 24 full-length clones, including IPL tumor suppressor gene, cyclophilin A, selenoprotein W, cystatin A, tryosyl-tRNA synthetase and calcineurin homologous protein. CONCLUSION These genes were mostly first reported in Dasyatis akajei. Further studies may partly explain the molecular mechanisms by which the caudal spine of Dasyatis akajei were often used in the treatment of inflammation, parasitosis and cancer in the traditional Chinese medicine.

Original languageEnglish (US)
Number of pages1
JournalYaoxue Xuebao
Volume36
Issue number12
StatePublished - Dec 1 2001
Externally publishedYes

Keywords

  • Caudal spine
  • Dasyatis akajei
  • cDNA expression library

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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    Tu, H. B., Wei, J. W., Jiang, X. Y., Chen, H. P., Zhong, X. F., Wu, W. Y., & Xu, A. L. (2001). Construction of cDNA expression library from the caudal spine of Dasyatis akajei. Yaoxue Xuebao, 36(12).