Construction of a multi RE module: Exploitation of mechanochemistry of restriction endonucleases

Pratul K. Agarwal, Sanjoy K. Bhattacharya

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We describe the construction of a multi-immobilized restriction endonuclease module (Multi RE module). We demonstrate that the applied mechanical stress enables modulation of enzyme activity and modulation of recognition site selectivity (in oligonucleotides of ≃200 bp) of immobilized restriction endonucleases. The central module which is consisted of different strips of immobilized restriction endonucleases allows limited digestion of a large DNA sample in a controlled manner as a function of applied mechanical stress on strips. The stress-activity relationship and the effect of repeated cycles of stress and relaxation on the immobilized strips are presented here.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalBiotechnology and Bioengineering
Volume65
Issue number2
DOIs
StatePublished - Oct 20 1999
Externally publishedYes

Keywords

  • Immobilized restricted enzymes
  • Multi RE module
  • Restriction endonucleases

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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