Construction of a mammalian transducing vector from the genome of Moloney murine leukemia virus

Eli Gilboa, M. Kolbe, K. Noonan, R. Kucherlapati

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A 0.9-kilobase DNA fragment from the genome of Moloney murine leukemia virus, including the viral long terminal repeat, was covalently linked to the herpes simplex virus I thymidine kinase (tk) gene whose promoter was previously removed. The hybrid DNA structure was introduced into the chromosome of tk- mouse cells at single copy numbers, via transfection procedures. Cells expressing the newly introduced tk gene were identified by the HAT selection procedure and analyzed for tk- and Moloney murine leukemia virus-specific DNA and RNA sequences by blot hybridization procedures. Expression of the tk gene is dependent on function(s) provided in cis by the viral DNA fragment. Vectors derived from this region are termed rGag (rG) vectors.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalJournal of Virology
Volume44
Issue number3
StatePublished - Dec 1 1982
Externally publishedYes

Fingerprint

Murine leukemia virus
Moloney murine leukemia virus
thymidine kinase
Thymidine Kinase
Genome
genome
DNA
Genes
nucleotide sequences
herpes simplex
terminal repeat sequences
genes
Terminal Repeat Sequences
Viral DNA
Simplexvirus
transfection
Transfection
hybridization
Chromosomes
promoter regions

ASJC Scopus subject areas

  • Immunology

Cite this

Construction of a mammalian transducing vector from the genome of Moloney murine leukemia virus. / Gilboa, Eli; Kolbe, M.; Noonan, K.; Kucherlapati, R.

In: Journal of Virology, Vol. 44, No. 3, 01.12.1982, p. 845-851.

Research output: Contribution to journalArticle

Gilboa, Eli ; Kolbe, M. ; Noonan, K. ; Kucherlapati, R. / Construction of a mammalian transducing vector from the genome of Moloney murine leukemia virus. In: Journal of Virology. 1982 ; Vol. 44, No. 3. pp. 845-851.
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