Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells

Yaron J. Lidor, Feng J. Xu, Otoniel Martínez-Maza, George J. Olt, Jeffrey R. Marks, Andrew Berchuck, Sundaram Ramakrishnan, Jonathan S. Berek, Robert C. Bast

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Normal and neoplastic epithelial cells produce growth factors that can affect cells from different lineages. Epithelial ovarian cancers produce M-CSF and IL-6. In the present study, production of these cytokines has been measured in the apparently normal epithelial cells from which epithelial ovarian neoplasms are thought to arise. Epithelial cells from the surface of premenopausal human ovaries were established in short-term cultures. The cells bound anti-cytokeratin antibodies and exhibited characteristic epithelial morphology by light and transmission electron microscopy. M-CSF and IL-6 were detected in supernatants from cultures of these cells, using assays specific for each factor. Cytokine levels were comparable to those in supernatants from ovarian and breast cancer cell lines. M-CSF expression could also be demonstrated by immunohistochemical analysis with specific rabbit heteroantiserum. Thus, M-CSF and IL-6 are produced constitutively by normal as well as by neoplastic ovarian epithelium.

Original languageEnglish (US)
Pages (from-to)332-339
Number of pages8
JournalExperimental Cell Research
Volume207
Issue number2
DOIs
StatePublished - Aug 1993
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells'. Together they form a unique fingerprint.

  • Cite this

    Lidor, Y. J., Xu, F. J., Martínez-Maza, O., Olt, G. J., Marks, J. R., Berchuck, A., Ramakrishnan, S., Berek, J. S., & Bast, R. C. (1993). Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells. Experimental Cell Research, 207(2), 332-339. https://doi.org/10.1006/excr.1993.1200