Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells

Yaron J. Lidor, Feng J. Xu, Otoniel Martínez-Maza, George J. Olt, Jeffrey R. Marks, Andrew Berchuck, Sundaram Ramakrishnan, Jonathan S. Berek, Robert C. Bast

Research output: Contribution to journalArticle

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Abstract

Normal and neoplastic epithelial cells produce growth factors that can affect cells from different lineages. Epithelial ovarian cancers produce M-CSF and IL-6. In the present study, production of these cytokines has been measured in the apparently normal epithelial cells from which epithelial ovarian neoplasms are thought to arise. Epithelial cells from the surface of premenopausal human ovaries were established in short-term cultures. The cells bound anti-cytokeratin antibodies and exhibited characteristic epithelial morphology by light and transmission electron microscopy. M-CSF and IL-6 were detected in supernatants from cultures of these cells, using assays specific for each factor. Cytokine levels were comparable to those in supernatants from ovarian and breast cancer cell lines. M-CSF expression could also be demonstrated by immunohistochemical analysis with specific rabbit heteroantiserum. Thus, M-CSF and IL-6 are produced constitutively by normal as well as by neoplastic ovarian epithelium.

Original languageEnglish (US)
Pages (from-to)332-339
Number of pages8
JournalExperimental Cell Research
Volume207
Issue number2
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Macrophage Colony-Stimulating Factor
Interleukin-6
Epithelial Cells
Ovarian Neoplasms
Cytokines
Glandular and Epithelial Neoplasms
Cell Lineage
Keratins
Transmission Electron Microscopy
Anti-Idiotypic Antibodies
Ovary
Intercellular Signaling Peptides and Proteins
Epithelium
Cell Culture Techniques
Breast Neoplasms
Rabbits
Light
Cell Line

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lidor, Y. J., Xu, F. J., Martínez-Maza, O., Olt, G. J., Marks, J. R., Berchuck, A., ... Bast, R. C. (1993). Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells. Experimental Cell Research, 207(2), 332-339. https://doi.org/10.1006/excr.1993.1200

Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells. / Lidor, Yaron J.; Xu, Feng J.; Martínez-Maza, Otoniel; Olt, George J.; Marks, Jeffrey R.; Berchuck, Andrew; Ramakrishnan, Sundaram; Berek, Jonathan S.; Bast, Robert C.

In: Experimental Cell Research, Vol. 207, No. 2, 01.01.1993, p. 332-339.

Research output: Contribution to journalArticle

Lidor, Yaron J. ; Xu, Feng J. ; Martínez-Maza, Otoniel ; Olt, George J. ; Marks, Jeffrey R. ; Berchuck, Andrew ; Ramakrishnan, Sundaram ; Berek, Jonathan S. ; Bast, Robert C. / Constitutive production of macrophage colony-stimulating factor and interleukin-6 by human ovarian surface epithelial Cells. In: Experimental Cell Research. 1993 ; Vol. 207, No. 2. pp. 332-339.
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