Consistent fractionation of 13C in nature and in the laboratory

Growth-rate effects in some haptophyte algae

Robert R. Bidigare, Amim Fluegge, Katherine H. Freeman, Kristi L. Hanson, John M. Hayes, David Hollander, John P. Jasper, Linda L. King, Edward A. Laws, Jeffrey Milder, Frank J Millero, Richard Pancost, Brian N. Popp, Paul A. Steinberg, Stuart G. Wakeham

Research output: Contribution to journalArticle

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Abstract

The carbon isotopic fractionation accompanying formation of biomass by alkenone-producing algae in natural marine environments varies systematically with the concentration of dissolved phosphate. Specifically, if the fractionation is expressed by ε≈ δ e - δ p, where δ e, and δ p are the δ 13C values for dissolved CO 2 and for algal biomass (determined by isotopic analysis of C 37 alkadienones), respectively, and if C e is the concentration of dissolved CO 2, μmol kg -1, then b = 38 +160*[PO 4], where [PO 4] is the concentration of dissolved phosphate, μM, and b = (25 - ε P)C ε. The correlation found between b and [PO 4] is due to effects linking nutrient levels to growth rates and cellular carbon budgets for alkenone-containing algae, most likely by trace-metal limitations on algal growth. The relationship reported here is characteristic of 39 samples (r 2 = 0.95) from the Santa Monica Basin (six different times during the annual cycle), the equatorial Pacific (boreal spring and fall cruises as well as during an iron-enrichment experiment), and the Peru upwelling zone. Points representative of samples from the Sargasso Sea ([PO 4] ≤ 0.1 μM) fall above the b = f[PO 4] line. Analysis of correlations expected between μ(growth rate), ε P, and C e shows that, for our entire data set, most variations in ε p result from variations in μ rather than C e. Accordingly, before concentrations of dissolved CO 2 can be estimated from isotopic fractionations, some means of accounting for variations in growth rate must be found, perhaps by drawing on relationships between [PO 4] and Cd/Ca ratios in shells of planktonic foraminifera.

Original languageEnglish (US)
Pages (from-to)279-292
Number of pages14
JournalGlobal Biogeochemical Cycles
Volume11
Issue number2
StatePublished - 1997

Fingerprint

Carbon Monoxide
Algae
Fractionation
alkenone
fractionation
alga
isotopic fractionation
Biomass
Carbon
Phosphates
phosphate
isotopic analysis
carbon budget
biomass
planktonic foraminifera
annual cycle
Nutrients
trace metal
marine environment
upwelling

ASJC Scopus subject areas

  • Global and Planetary Change
  • Atmospheric Science
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Bidigare, R. R., Fluegge, A., Freeman, K. H., Hanson, K. L., Hayes, J. M., Hollander, D., ... Wakeham, S. G. (1997). Consistent fractionation of 13C in nature and in the laboratory: Growth-rate effects in some haptophyte algae. Global Biogeochemical Cycles, 11(2), 279-292.

Consistent fractionation of 13C in nature and in the laboratory : Growth-rate effects in some haptophyte algae. / Bidigare, Robert R.; Fluegge, Amim; Freeman, Katherine H.; Hanson, Kristi L.; Hayes, John M.; Hollander, David; Jasper, John P.; King, Linda L.; Laws, Edward A.; Milder, Jeffrey; Millero, Frank J; Pancost, Richard; Popp, Brian N.; Steinberg, Paul A.; Wakeham, Stuart G.

In: Global Biogeochemical Cycles, Vol. 11, No. 2, 1997, p. 279-292.

Research output: Contribution to journalArticle

Bidigare, RR, Fluegge, A, Freeman, KH, Hanson, KL, Hayes, JM, Hollander, D, Jasper, JP, King, LL, Laws, EA, Milder, J, Millero, FJ, Pancost, R, Popp, BN, Steinberg, PA & Wakeham, SG 1997, 'Consistent fractionation of 13C in nature and in the laboratory: Growth-rate effects in some haptophyte algae', Global Biogeochemical Cycles, vol. 11, no. 2, pp. 279-292.
Bidigare RR, Fluegge A, Freeman KH, Hanson KL, Hayes JM, Hollander D et al. Consistent fractionation of 13C in nature and in the laboratory: Growth-rate effects in some haptophyte algae. Global Biogeochemical Cycles. 1997;11(2):279-292.
Bidigare, Robert R. ; Fluegge, Amim ; Freeman, Katherine H. ; Hanson, Kristi L. ; Hayes, John M. ; Hollander, David ; Jasper, John P. ; King, Linda L. ; Laws, Edward A. ; Milder, Jeffrey ; Millero, Frank J ; Pancost, Richard ; Popp, Brian N. ; Steinberg, Paul A. ; Wakeham, Stuart G. / Consistent fractionation of 13C in nature and in the laboratory : Growth-rate effects in some haptophyte algae. In: Global Biogeochemical Cycles. 1997 ; Vol. 11, No. 2. pp. 279-292.
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abstract = "The carbon isotopic fractionation accompanying formation of biomass by alkenone-producing algae in natural marine environments varies systematically with the concentration of dissolved phosphate. Specifically, if the fractionation is expressed by ε≈ δ e - δ p, where δ e, and δ p are the δ 13C values for dissolved CO 2 and for algal biomass (determined by isotopic analysis of C 37 alkadienones), respectively, and if C e is the concentration of dissolved CO 2, μmol kg -1, then b = 38 +160*[PO 4], where [PO 4] is the concentration of dissolved phosphate, μM, and b = (25 - ε P)C ε. The correlation found between b and [PO 4] is due to effects linking nutrient levels to growth rates and cellular carbon budgets for alkenone-containing algae, most likely by trace-metal limitations on algal growth. The relationship reported here is characteristic of 39 samples (r 2 = 0.95) from the Santa Monica Basin (six different times during the annual cycle), the equatorial Pacific (boreal spring and fall cruises as well as during an iron-enrichment experiment), and the Peru upwelling zone. Points representative of samples from the Sargasso Sea ([PO 4] ≤ 0.1 μM) fall above the b = f[PO 4] line. Analysis of correlations expected between μ(growth rate), ε P, and C e shows that, for our entire data set, most variations in ε p result from variations in μ rather than C e. Accordingly, before concentrations of dissolved CO 2 can be estimated from isotopic fractionations, some means of accounting for variations in growth rate must be found, perhaps by drawing on relationships between [PO 4] and Cd/Ca ratios in shells of planktonic foraminifera.",
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AU - Hanson, Kristi L.

AU - Hayes, John M.

AU - Hollander, David

AU - Jasper, John P.

AU - King, Linda L.

AU - Laws, Edward A.

AU - Milder, Jeffrey

AU - Millero, Frank J

AU - Pancost, Richard

AU - Popp, Brian N.

AU - Steinberg, Paul A.

AU - Wakeham, Stuart G.

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N2 - The carbon isotopic fractionation accompanying formation of biomass by alkenone-producing algae in natural marine environments varies systematically with the concentration of dissolved phosphate. Specifically, if the fractionation is expressed by ε≈ δ e - δ p, where δ e, and δ p are the δ 13C values for dissolved CO 2 and for algal biomass (determined by isotopic analysis of C 37 alkadienones), respectively, and if C e is the concentration of dissolved CO 2, μmol kg -1, then b = 38 +160*[PO 4], where [PO 4] is the concentration of dissolved phosphate, μM, and b = (25 - ε P)C ε. The correlation found between b and [PO 4] is due to effects linking nutrient levels to growth rates and cellular carbon budgets for alkenone-containing algae, most likely by trace-metal limitations on algal growth. The relationship reported here is characteristic of 39 samples (r 2 = 0.95) from the Santa Monica Basin (six different times during the annual cycle), the equatorial Pacific (boreal spring and fall cruises as well as during an iron-enrichment experiment), and the Peru upwelling zone. Points representative of samples from the Sargasso Sea ([PO 4] ≤ 0.1 μM) fall above the b = f[PO 4] line. Analysis of correlations expected between μ(growth rate), ε P, and C e shows that, for our entire data set, most variations in ε p result from variations in μ rather than C e. Accordingly, before concentrations of dissolved CO 2 can be estimated from isotopic fractionations, some means of accounting for variations in growth rate must be found, perhaps by drawing on relationships between [PO 4] and Cd/Ca ratios in shells of planktonic foraminifera.

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