Conserved MHC class I peptide binding motif between humans and rhesus macaques

John L. Dzuris, John Sidney, Ettore Appella, Robert W. Chesnut, David Watkins, Alessandro Sette

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Since the onset of the HIV pandemic, the use of nonhuman primate models of infection has increasingly become important. An excellent model to study HIV infection and immunological responses, in particular cell-mediated immune responses, is SIV infection of rhesus macaques. CTL epitopes have been mapped using SIV-infected rhesus macaques, but, to date, a peptide binding motif has been described for only one rhesus class I MHC molecule, Mamu-A*01. Herein, we have established peptide-live cell binding assays for four rhesus MHC class I molecules: Mamu-A*11, -B*03, -B*04, and -B*17. Using such assays, peptide binding motifs have been established for all four of these rhesus MHC class I molecules. With respect to the nature and spacing of crucial anchor positions, the motifs defined for Mamu-B*04 and -B*17 present unique features not previously observed for other primate species. The motifs identified for Mamu-A*11 and -B*03 are very similar to the peptide binding motifs previously described for human HLA-B*44 and -B*27, respectively. Accordingly, naturally processed peptides derived from HLA-B*44 and HLA- B*27 specifically bind Mamu-A*11 and Mamu-B*03, respectively, indicating that conserved MHC class I binding capabilities exist between rhesus macaques and humans. The definition of four rhesus MHC class I-specific motifs expands our ability to accurately detect and quantitate immune responses to MHC class I-restricted epitopes in rhesus macaques and to rationally design peptide epitope-based model vaccine constructs destined for use in nonhuman primates.

Original languageEnglish
Pages (from-to)283-291
Number of pages9
JournalJournal of Immunology
Volume164
Issue number1
StatePublished - Jan 1 2000
Externally publishedYes

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Macaca mulatta
Peptides
HLA-B Antigens
Primates
Epitopes
Pandemics
Infection
HIV Infections
Vaccines
HIV

ASJC Scopus subject areas

  • Immunology

Cite this

Dzuris, J. L., Sidney, J., Appella, E., Chesnut, R. W., Watkins, D., & Sette, A. (2000). Conserved MHC class I peptide binding motif between humans and rhesus macaques. Journal of Immunology, 164(1), 283-291.

Conserved MHC class I peptide binding motif between humans and rhesus macaques. / Dzuris, John L.; Sidney, John; Appella, Ettore; Chesnut, Robert W.; Watkins, David; Sette, Alessandro.

In: Journal of Immunology, Vol. 164, No. 1, 01.01.2000, p. 283-291.

Research output: Contribution to journalArticle

Dzuris, JL, Sidney, J, Appella, E, Chesnut, RW, Watkins, D & Sette, A 2000, 'Conserved MHC class I peptide binding motif between humans and rhesus macaques', Journal of Immunology, vol. 164, no. 1, pp. 283-291.
Dzuris JL, Sidney J, Appella E, Chesnut RW, Watkins D, Sette A. Conserved MHC class I peptide binding motif between humans and rhesus macaques. Journal of Immunology. 2000 Jan 1;164(1):283-291.
Dzuris, John L. ; Sidney, John ; Appella, Ettore ; Chesnut, Robert W. ; Watkins, David ; Sette, Alessandro. / Conserved MHC class I peptide binding motif between humans and rhesus macaques. In: Journal of Immunology. 2000 ; Vol. 164, No. 1. pp. 283-291.
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