Conjugate addition reactions combined with free-radical cross-linking for the design of materials for tissue engineering

D. L. Elbert, J. A. Hubbell

Research output: Contribution to journalArticle

324 Citations (Scopus)

Abstract

PEG-diacrylamide was synthesized and utilized to make materials for tissue engineering. Acrylamide groups readily react with thiol groups, and peptides containing a single thiol group were coupled to the PEG-diacrylamide in aqueous solution at room temperature in about 2 h. If only a portion of the acrylamide groups were targeted for reaction with peptide, sufficient amounts of PEG-diacrylamide remained to be polymerized by free-radical mechanisms via photoinitiation. The photopolymerization step can be performed in contact with cells, providing a means to produce bioactive scaffolds for tissue engineering. The photopolymerization conditions and precursor composition greatly affect the stiffness of the materials, which subsequently affected cell spreading. The kinetics and extent of cell spreading on the bioactive materials were measured and compared to cell spreading on tissue culture polystyrene. Although the PEG materials resist protein adsorption, the experiments suggest that the cells can secrete extracellular matrix that can adhere to the gels.

Original languageEnglish
Pages (from-to)430-441
Number of pages12
JournalBiomacromolecules
Volume2
Issue number2
DOIs
StatePublished - Aug 15 2001
Externally publishedYes

Fingerprint

Addition reactions
Tissue Engineering
Free radicals
Tissue engineering
Polyethylene glycols
Free Radicals
Photopolymerization
Acrylamide
Sulfhydryl Compounds
Peptides
Tissue culture
Polystyrenes
Scaffolds (biology)
Gels
Stiffness
Adsorption
Extracellular Matrix
Proteins
Kinetics
Chemical analysis

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Conjugate addition reactions combined with free-radical cross-linking for the design of materials for tissue engineering. / Elbert, D. L.; Hubbell, J. A.

In: Biomacromolecules, Vol. 2, No. 2, 15.08.2001, p. 430-441.

Research output: Contribution to journalArticle

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