Conformational and receptor binding properties of human EGF and TGF-alpha second loop fragments.

K. H. Han, J. A. Ferretti, C. H. Niu, V. Lokeshwar, R. Clarke, D. Katz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The solution conformation of the second loop fragment of human EGF, [Ala20] EGF (14-31), was determined using two-dimensional NMR homonuclear Hartmann-Hahn and rotating frame nuclear Overhauser enhancement spectroscopy. The results are compared with the conformation of the second loop fragment of human TGF-alpha, [Ala21] TGF-alpha(16-32), and with that of the second loop of intact EGF. Comparison of the two experimentally determined structures of the second loop fragments shows significant differences in the turn regions of each peptide. For the EGF fragment, hydrophobic side chain groups protrude away from the ring, whereas for the TGF-alpha fragment hydrophilic groups are directed away from the ring. Although these turn regions represent the putative receptor binding sites, neither second loop fragment binds to the EGF receptor. The biological activity is discussed in terms of the conformational differences found for the two second loop fragments.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalJournal of molecular recognition : JMR
Volume1
Issue number3
StatePublished - Jun 1 1988
Externally publishedYes

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Transforming Growth Factor alpha
Epidermal Growth Factor
Conformations
Bioactivity
Epidermal Growth Factor Receptor
Spectrum Analysis
Binding Sites
Nuclear magnetic resonance
Spectroscopy
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Han, K. H., Ferretti, J. A., Niu, C. H., Lokeshwar, V., Clarke, R., & Katz, D. (1988). Conformational and receptor binding properties of human EGF and TGF-alpha second loop fragments. Journal of molecular recognition : JMR, 1(3), 116-123.

Conformational and receptor binding properties of human EGF and TGF-alpha second loop fragments. / Han, K. H.; Ferretti, J. A.; Niu, C. H.; Lokeshwar, V.; Clarke, R.; Katz, D.

In: Journal of molecular recognition : JMR, Vol. 1, No. 3, 01.06.1988, p. 116-123.

Research output: Contribution to journalArticle

Han, KH, Ferretti, JA, Niu, CH, Lokeshwar, V, Clarke, R & Katz, D 1988, 'Conformational and receptor binding properties of human EGF and TGF-alpha second loop fragments.', Journal of molecular recognition : JMR, vol. 1, no. 3, pp. 116-123.
Han, K. H. ; Ferretti, J. A. ; Niu, C. H. ; Lokeshwar, V. ; Clarke, R. ; Katz, D. / Conformational and receptor binding properties of human EGF and TGF-alpha second loop fragments. In: Journal of molecular recognition : JMR. 1988 ; Vol. 1, No. 3. pp. 116-123.
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AU - Clarke, R.

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