Abstract
The surface chemistry and spectroscopy of the reduced lysozyme Langmuir monolayer were investigated at different pH values to compare with the native one. It was found that the limiting molecular area of the reduced lysozyme was not subphase pH dependent as the native lysozyme. To explain this result in terms of the conformation and orientation of the lysozyme Langmuir monolayer at various subphase pH values, we have used infrared reflection absorption spectroscopy. The interpretation of the results make plausible change of the conformation and orientation of the native lysozyme Langmuir monolayer with the subphase pH 3, 6, and 11.
| Original language | English |
|---|---|
| Pages (from-to) | 2842-2849 |
| Number of pages | 8 |
| Journal | Langmuir |
| Volume | 25 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 3 2009 |
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ASJC Scopus subject areas
- Electrochemistry
- Condensed Matter Physics
- Surfaces and Interfaces
- Materials Science(all)
- Spectroscopy
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Conformation of lysozyme langmuir monolayer studied by infrared reflection absorption spectroscopy. / Thakur, Garima; Leblanc, Roger.
In: Langmuir, Vol. 25, No. 5, 03.03.2009, p. 2842-2849.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Conformation of lysozyme langmuir monolayer studied by infrared reflection absorption spectroscopy
AU - Thakur, Garima
AU - Leblanc, Roger
PY - 2009/3/3
Y1 - 2009/3/3
N2 - The surface chemistry and spectroscopy of the reduced lysozyme Langmuir monolayer were investigated at different pH values to compare with the native one. It was found that the limiting molecular area of the reduced lysozyme was not subphase pH dependent as the native lysozyme. To explain this result in terms of the conformation and orientation of the lysozyme Langmuir monolayer at various subphase pH values, we have used infrared reflection absorption spectroscopy. The interpretation of the results make plausible change of the conformation and orientation of the native lysozyme Langmuir monolayer with the subphase pH 3, 6, and 11.
AB - The surface chemistry and spectroscopy of the reduced lysozyme Langmuir monolayer were investigated at different pH values to compare with the native one. It was found that the limiting molecular area of the reduced lysozyme was not subphase pH dependent as the native lysozyme. To explain this result in terms of the conformation and orientation of the lysozyme Langmuir monolayer at various subphase pH values, we have used infrared reflection absorption spectroscopy. The interpretation of the results make plausible change of the conformation and orientation of the native lysozyme Langmuir monolayer with the subphase pH 3, 6, and 11.
UR - http://www.scopus.com/inward/record.url?scp=65249147077&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65249147077&partnerID=8YFLogxK
U2 - 10.1021/la803233p
DO - 10.1021/la803233p
M3 - Article
C2 - 19437700
AN - SCOPUS:65249147077
VL - 25
SP - 2842
EP - 2849
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 5
ER -