Conformal barrier by interfacial polymerization in contact with cells and tissue

A. S. Sawhney, C. P. Pathak, P. R. Cox, J. A. Hubbell

Research output: Contribution to journalConference article

Abstract

We have previously described the rapid photopolymerization of water soluble polyethylene glycol based macromers using visible lasers in contact with tissue and cells. These polymerizations proceed throughout the bulk of the macromer solution. Here we describe a technique whereby this polymerization can be directly initiated form the tissue or cell surface itself and be confined to the interface between the polymerizing from and the bulk macromer solution. The effect of various treatments on the viability of HFF cells are summarized. In evaluating the effect of photopolymerization on cell viability, the objective was to compare the interfacial scheme of photopolymerization to the scheme in which the dye was present in the bulk. The interfacial approach was seen to have some but not a strong effect on cell viability. This effect was not significantly different (p<0.05, unpaired test) over the LWUV induced polymerization for the PEG-MA. Visible polymerization using a commercial low molecular weight PEG diacrylate (400 DA) significantly decreased cell viability over control (Treatment 9). The individual components of the initiating system were not found to have any effect on cell viability. In conclusion we have examined a technique interfacial polymerization In some cases the temporary presence of these barriers is desirable. We have described photopolymerizable macromers, which can from hydrolytically degradable hydrogels.

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ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics

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