Conformal barrier by interfacial polymerization in contact with cells and tissue

A. S. Sawhney, C. P. Pathak, P. R. Cox, J. A. Hubbell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have previously described the rapid photopolymerization of water soluble polyethylene glycol based macromers using visible lasers in contact with tissue and cells. These polymerizations proceed throughout the bulk of the macromer solution. Here we describe a technique whereby this polymerization can be directly initiated form the tissue or cell surface itself and be confined to the interface between the polymerizing from and the bulk macromer solution. The effect of various treatments on the viability of HFF cells are summarized. In evaluating the effect of photopolymerization on cell viability, the objective was to compare the interfacial scheme of photopolymerization to the scheme in which the dye was present in the bulk. The interfacial approach was seen to have some but not a strong effect on cell viability. This effect was not significantly different (p<0.05, unpaired test) over the LWUV induced polymerization for the PEG-MA. Visible polymerization using a commercial low molecular weight PEG diacrylate (400 DA) significantly decreased cell viability over control (Treatment 9). The individual components of the initiating system were not found to have any effect on cell viability. In conclusion we have examined a technique interfacial polymerization In some cases the temporary presence of these barriers is desirable. We have described photopolymerizable macromers, which can from hydrolytically degradable hydrogels.

Original languageEnglish (US)
Title of host publicationPolymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering
Editors Anon
PublisherPubl by ACS
Pages526
Number of pages1
Volume69
StatePublished - 1993
Externally publishedYes
EventProceedings of the American Chemical Society Division of Polymeric Materials - Science and Engineering - Chicago, IL, USA
Duration: Jun 11 1993Jun 11 1993

Other

OtherProceedings of the American Chemical Society Division of Polymeric Materials - Science and Engineering
CityChicago, IL, USA
Period6/11/936/11/93

Fingerprint

Polymerization
Tissue
Photopolymerization
Cells
Polyethylene glycols
Hydrogels
Coloring Agents
Dyes
Molecular weight
Water
Lasers

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Polymers and Plastics

Cite this

Sawhney, A. S., Pathak, C. P., Cox, P. R., & Hubbell, J. A. (1993). Conformal barrier by interfacial polymerization in contact with cells and tissue. In Anon (Ed.), Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering (Vol. 69, pp. 526). Publ by ACS.

Conformal barrier by interfacial polymerization in contact with cells and tissue. / Sawhney, A. S.; Pathak, C. P.; Cox, P. R.; Hubbell, J. A.

Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. ed. / Anon. Vol. 69 Publ by ACS, 1993. p. 526.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sawhney, AS, Pathak, CP, Cox, PR & Hubbell, JA 1993, Conformal barrier by interfacial polymerization in contact with cells and tissue. in Anon (ed.), Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. vol. 69, Publ by ACS, pp. 526, Proceedings of the American Chemical Society Division of Polymeric Materials - Science and Engineering, Chicago, IL, USA, 6/11/93.
Sawhney AS, Pathak CP, Cox PR, Hubbell JA. Conformal barrier by interfacial polymerization in contact with cells and tissue. In Anon, editor, Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. Vol. 69. Publ by ACS. 1993. p. 526
Sawhney, A. S. ; Pathak, C. P. ; Cox, P. R. ; Hubbell, J. A. / Conformal barrier by interfacial polymerization in contact with cells and tissue. Polymeric Materials Science and Engineering, Proceedings of the ACS Division of Polymeric Materials Science and Engineering. editor / Anon. Vol. 69 Publ by ACS, 1993. pp. 526
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