Concurrence of chromosome 3 and 4 aberrations in human uveal melanoma

Eva Sipos, Kata Hegyi, Andrea Treszl, Zita Steiber, Gabor Mehes, Nikoletta Dobos, Klara Fodor, Gabor Olah, Lorant Szekvolgyi, Andrew V. Schally, Gabor Halmos

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Uveal melanoma (UM) is the most common primary intraocular malignancy with a very poor prognosis. The most frequent chromosome aberration in UM is the monosomy of chromosome 3. Previously, we demonstrated that ~50% of UMs express type-I receptor for luteinizing hormone-releasing hormone (LH-RH-R). The gene encoding LH-RH-R is located in chromosome 4 (location: 4q21.2); however, the occurrence of numerical aberrations of chromosome 4 have never been studied in UM. In the present study, we investigated the abnormalities of chromosome 3 and 4, and the possible correlation between them, as well as with LH-RH-R expression. Forty-six specimens of UM were obtained after enucleation. Numerical aberrations of chromosome 3 and 4 were studied by fluorescence in situ hybridization (FISH). Chromosome 4 was detected in normal biparental disomy only in 14 (30%) samples; however, 32 cases (70%) showed more than 2 signals/nucleus. Monosomy of chromosome 3 could be found in 16 (35%) samples. In 6 specimens (13%), more than 2 copies of chromosome 3 were found, while normal biparental disomy was detected in 24 (52%) samples. Statistical analysis indicated a statistically significant (p<0.05) correlation between the copy number of chromosome 3 and 4. Moreover, moderate difference was revealed in the survival rate of the UM patients with various pathological profiles. No correlation was found between chromosome aberrations and LH-RH-R expression. Our results clearly demonstrate abnormalities in chromosome 3 and 4 and the incidence of the monosomy of chromosome 3 in human UM. In summary, our results provide new incite concerning the genetic background of this tumor. Our findings could contribute to a more precise determination of the prognosis of human UM and to the development of new therapeutic approaches to this malignancy.

Original languageEnglish (US)
Pages (from-to)1927-1934
Number of pages8
JournalOncology Reports
Volume37
Issue number4
DOIs
StatePublished - Apr 2017

Keywords

  • Aberrations of chromosome 3 and 4
  • Chromosome index
  • Fluorescence in situ hybridization
  • Human uveal melanoma
  • Luteinizing hormone-releasing hormone receptor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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