Concentration of Corticotropin Releasing Factor by Chromatography on Carboxymethylcellulose

Andrew V. Schally, Roger Guillemin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

1) Neurohypophysial extracts of commercial origin were chromatographed on carboxymethylcellulose cation exchange adsorbent under conditions of pH and ionic strength gradient in view of separating CRF. An in vivo assay was used to assess CRF activity. 2) The method described is useful in large scale preparations. Fractions containing CRF were regularly found to emerge before lysine vasopressin and were well separated from oxytocin.

Original languageEnglish (US)
Pages (from-to)138-139
Number of pages2
JournalProceedings of the Society for Experimental Biology and Medicine
Volume100
Issue number1
DOIs
StatePublished - Jan 1959
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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