Concentration of Corticotropin Releasing Factor by Chromatography on Carboxymethylcellulose

Andrew V. Schally; Roger Guillemin

doi:10.3181/00379727-100-24551

Concentration of Corticotropin Releasing Factor by Chromatography on Carboxymethylcellulose

Andrew V. Schally, Roger Guillemin

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

1) Neurohypophysial extracts of commercial origin were chromatographed on carboxymethylcellulose cation exchange adsorbent under conditions of pH and ionic strength gradient in view of separating CRF. An in vivo assay was used to assess CRF activity. 2) The method described is useful in large scale preparations. Fractions containing CRF were regularly found to emerge before lysine vasopressin and were well separated from oxytocin.

Original language	English (US)
Pages (from-to)	138-139
Number of pages	2
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	100
Issue number	1
DOIs	https://doi.org/10.3181/00379727-100-24551
State	Published - Jan 1959
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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title = "Concentration of Corticotropin Releasing Factor by Chromatography on Carboxymethylcellulose",

abstract = "1) Neurohypophysial extracts of commercial origin were chromatographed on carboxymethylcellulose cation exchange adsorbent under conditions of pH and ionic strength gradient in view of separating CRF. An in vivo assay was used to assess CRF activity. 2) The method described is useful in large scale preparations. Fractions containing CRF were regularly found to emerge before lysine vasopressin and were well separated from oxytocin.",

author = "Schally, {Andrew V.} and Roger Guillemin",

year = "1959",

month = jan,

doi = "10.3181/00379727-100-24551",

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AU - Guillemin, Roger

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AB - 1) Neurohypophysial extracts of commercial origin were chromatographed on carboxymethylcellulose cation exchange adsorbent under conditions of pH and ionic strength gradient in view of separating CRF. An in vivo assay was used to assess CRF activity. 2) The method described is useful in large scale preparations. Fractions containing CRF were regularly found to emerge before lysine vasopressin and were well separated from oxytocin.

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