Computerized image analysis of lung expansion patterns in surfactant treated immature newborn rabbits

Per Berggren, Jean Paul Rigaut, Tore Curstedt, Bengt Robertson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study evaluates the alveolar stereological profile of immature rabbit lungs after treatment with various surfactant preparations, deliberately modified to represent different rates of film adsorption, minimum surface tension and compressibility. Surfactant was isolated from porcine or bovine lungs, and some of these preparations were enriched with dipalmitoylphosphatidylcholine and other synthetic lipids. Alveolar stereological parameters were evaluated in histological lung sections by computerized interactive image analysis. Surfactant treatment enhanced lung expansion, with a significant correlation coefficient between dynamic compliance and alveolar volume density in all groups of animals. Surfactant treatment also significantly increased alveolar image area. Enrichment of surfactant with synthetic lipids did not improve stereological parameters, but caused a significant increase in the coefficient of variation for alveolar perimeter and image area, suggesting more heterogeneous expansion pattern. Experimental evaluation of exogenous surfactants in immature newborn animals should include assessment of alveolar stereological parameters to detect deviations from the uniform expansion pattern seen after treatment with an optimal surfactant substitute. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)45-53
Number of pages9
JournalRespiration Physiology
Volume115
Issue number1
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Surface-Active Agents
Rabbits
Lung
Newborn Animals
Lipids
1,2-Dipalmitoylphosphatidylcholine
Surface Tension
Adsorption
Compliance
Swine

Keywords

  • Development, surfactant, immature lung
  • Mammals, rabbit
  • Mechanics of breathing, surfactant
  • Surfactant, immature lung

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Computerized image analysis of lung expansion patterns in surfactant treated immature newborn rabbits. / Berggren, Per; Paul Rigaut, Jean; Curstedt, Tore; Robertson, Bengt.

In: Respiration Physiology, Vol. 115, No. 1, 01.01.1999, p. 45-53.

Research output: Contribution to journalArticle

Berggren, Per ; Paul Rigaut, Jean ; Curstedt, Tore ; Robertson, Bengt. / Computerized image analysis of lung expansion patterns in surfactant treated immature newborn rabbits. In: Respiration Physiology. 1999 ; Vol. 115, No. 1. pp. 45-53.
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