TY - JOUR
T1 - Comprehensive analysis of Hox gene expression in the amphipod crustacean Parhyale hawaiensis
AU - Serano, Julia M.
AU - Martin, Arnaud
AU - Liubicich, Danielle M.
AU - Jarvis, Erin
AU - Bruce, Heather S.
AU - La, Konnor
AU - Browne, William E.
AU - Grimwood, Jane
AU - Patel, Nipam H.
N1 - Funding Information:
We thank Ron Parchem for the Parhyale en1 clone, Jay Rehm for thoughtful discussions regarding early germband cell divisions in Parhyale , Rachel Thayer for the abd-A in situ in Fig. 2 , and Rob White for providing the FP6.87 monoclonal antibody. The BAC sequencing work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under Contract no. DE-AC02-05CH11231 . This work was supported by NSF Grant IOS-1257379 to NHP.
PY - 2016
Y1 - 2016
N2 - Hox genes play crucial roles in establishing regional identity along the anterior-posterior axis in bilaterian animals, and have been implicated in generating morphological diversity throughout evolution. Here we report the identification, expression, and initial genomic characterization of the complete set of Hox genes from the amphipod crustacean Parhyale hawaiensis. Parhyale is an emerging model system that is amenable to experimental manipulations and evolutionary comparisons among the arthropods. Our analyses indicate that the Parhyale genome contains a single copy of each canonical Hox gene with the exception of fushi tarazu, and preliminary mapping suggests that at least some of these genes are clustered together in the genome. With few exceptions, Parhyale Hox genes exhibit both temporal and spatial colinearity, and expression boundaries correlate with morphological differences between segments and their associated appendages. This work represents the most comprehensive analysis of Hox gene expression in a crustacean to date, and provides a foundation for functional studies aimed at elucidating the role of Hox genes in arthropod development and evolution.
AB - Hox genes play crucial roles in establishing regional identity along the anterior-posterior axis in bilaterian animals, and have been implicated in generating morphological diversity throughout evolution. Here we report the identification, expression, and initial genomic characterization of the complete set of Hox genes from the amphipod crustacean Parhyale hawaiensis. Parhyale is an emerging model system that is amenable to experimental manipulations and evolutionary comparisons among the arthropods. Our analyses indicate that the Parhyale genome contains a single copy of each canonical Hox gene with the exception of fushi tarazu, and preliminary mapping suggests that at least some of these genes are clustered together in the genome. With few exceptions, Parhyale Hox genes exhibit both temporal and spatial colinearity, and expression boundaries correlate with morphological differences between segments and their associated appendages. This work represents the most comprehensive analysis of Hox gene expression in a crustacean to date, and provides a foundation for functional studies aimed at elucidating the role of Hox genes in arthropod development and evolution.
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U2 - 10.1016/j.ydbio.2015.10.029
DO - 10.1016/j.ydbio.2015.10.029
M3 - Article
C2 - 26569556
AN - SCOPUS:84947335358
VL - 409
SP - 297
EP - 309
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -