Composition of human islet cell preparations for transplantation

C. E. Sever, A. J. Demetris, J. Zeng, P. Carroll, A. Tzakis, J. J. Fung, T. E. Starzl, Camillo Ricordi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To study the cellular composition of human islet cell isolates for transplantation, formalin-fixed and paraffin-embedded cell pellets were stained by the immunoperoxidase method with a panel of antibodies characterising endocrine, epithelial, soft tissue and haematolymphoid components. Immediately after separation, the isolates contained 30-80% islet cells, differing mainly in the content of islet and acinar cells, whereas the soft tissue, ductal/ductular and haematolymphoid elements comprised a relatively constant 10-20%. After 1 week in culture the islet cell content of less highly purified isolates (30-40% islets) dropped dramatically to 5%. The highly purified isolates (70-80% islets) showed only a minimal change in cellular composition; however, approximately two-thirds of islet cells were degranulated and did not stain for insulin. Haematolymphoid components were still present in all cultured isolates. We conclude that primarily mechanical purification methods and short-term culture are not sufficient to eliminate highly immunogenic cells. In addition, short-term culture is deleterious to the isolate if a significant number of acinar cells is still present after enrichment.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalActa Diabetologica
Volume28
Issue number3-4
DOIs
StatePublished - Sep 1 1992
Externally publishedYes

Fingerprint

Islets of Langerhans Transplantation
Cell Transplantation
Islets of Langerhans
Acinar Cells
Paraffin
Formaldehyde
Coloring Agents
Epithelium
Insulin
Antibodies

Keywords

  • Cell isolation
  • Immunoperoxidase techniques
  • Islets of Langerhand
  • Transplantation

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Sever, C. E., Demetris, A. J., Zeng, J., Carroll, P., Tzakis, A., Fung, J. J., ... Ricordi, C. (1992). Composition of human islet cell preparations for transplantation. Acta Diabetologica, 28(3-4), 233-238. https://doi.org/10.1007/BF00779005

Composition of human islet cell preparations for transplantation. / Sever, C. E.; Demetris, A. J.; Zeng, J.; Carroll, P.; Tzakis, A.; Fung, J. J.; Starzl, T. E.; Ricordi, Camillo.

In: Acta Diabetologica, Vol. 28, No. 3-4, 01.09.1992, p. 233-238.

Research output: Contribution to journalArticle

Sever, CE, Demetris, AJ, Zeng, J, Carroll, P, Tzakis, A, Fung, JJ, Starzl, TE & Ricordi, C 1992, 'Composition of human islet cell preparations for transplantation', Acta Diabetologica, vol. 28, no. 3-4, pp. 233-238. https://doi.org/10.1007/BF00779005
Sever CE, Demetris AJ, Zeng J, Carroll P, Tzakis A, Fung JJ et al. Composition of human islet cell preparations for transplantation. Acta Diabetologica. 1992 Sep 1;28(3-4):233-238. https://doi.org/10.1007/BF00779005
Sever, C. E. ; Demetris, A. J. ; Zeng, J. ; Carroll, P. ; Tzakis, A. ; Fung, J. J. ; Starzl, T. E. ; Ricordi, Camillo. / Composition of human islet cell preparations for transplantation. In: Acta Diabetologica. 1992 ; Vol. 28, No. 3-4. pp. 233-238.
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