Component analysis of the fluorescence spectra of a lignin model compound

Ksenija Radotić, Aleksandar Kalauzi, Daniela Djikanović, Milorad Jeremić, Roger M. Leblanc, Zoran G. Cerović

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

In order to test whether lignin fluorescence originates from discrete fluorophores, fluorescence emission spectra of the lignin model dehydrogenative polymer (DHP) were analyzed by the band deconvolution method and time-resolved analysis of both the excitation and emission spectra. Two series of 22 fluorescence emission spectra of DHP in chloroform/methanol (3:1, v/v) solution, and as a solid suspension in water, were deconvoluted into three fluorescence and one Raman Gaussian components. Emission spectra were obtained by stepwise variation of the excitation wavelength from 360 to 465 nm. Deconvolution was performed by nonlinear fitting of all three Gaussian parameters: area, width and position. Position of all components in a series was treated as a random variable and its approximate probability distribution (APD) calculated from a series of histograms with increasing number of abscissa intervals. A five peak multimodal APD profile was obtained for both series of DHP emission spectra. The mean fluorescence lifetime varied with wavelength both in the emission and the excitation decay-associated spectra (DAS), where four kinetic components were resolved. The shapes of the excitation spectra of the four components were quite different and gradually shifted bathochromically. The multicomponent nature of the DHP emission spectra along with the changes in the mean fluorescence lifetime and the form of the excitation DAS of the four components give evidence of the heterogeneous origin of fluorescent species emitting in the visible.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Photochemistry and Photobiology B: Biology
Volume83
Issue number1
DOIs
StatePublished - Apr 3 2006

Keywords

  • Blue-green fluorescence
  • Fluorophores
  • Gaussian components
  • Lignin model compound
  • Nonlinear fitting
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

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