Complex of aminoacyl-transfer RNA synthetases

Alok Kumar Bandyopadhyay, Murray P Deutscher

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

A high molecular weight complex has been isolated from rat liver which contains all the aminoacyl-transfer RNA synthetases and and a large proportion of the tRNA present in the high-speed supernatant fraction. The complex could be partially purified by chromatography on Sephadex G200 and Sepharose 6B. The complex could be disrupted by excess homogenization and freezing and thawing with a concomitant decrease in the activities of the aminoacyl-tRNA synthetases. The results suggest that some of the presumed "soluble" components of the protein synthetic apparatus may be present in highly organized structures within the cell.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalJournal of Molecular Biology
Volume60
Issue number1
DOIs
StatePublished - Aug 28 1971
Externally publishedYes

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Amino Acyl-tRNA Synthetases
Transfer RNA
Sepharose
Freezing
Chromatography
Molecular Weight
Liver
Proteins
sephadex

ASJC Scopus subject areas

  • Virology

Cite this

Complex of aminoacyl-transfer RNA synthetases. / Bandyopadhyay, Alok Kumar; Deutscher, Murray P.

In: Journal of Molecular Biology, Vol. 60, No. 1, 28.08.1971, p. 113-122.

Research output: Contribution to journalArticle

Bandyopadhyay, Alok Kumar ; Deutscher, Murray P. / Complex of aminoacyl-transfer RNA synthetases. In: Journal of Molecular Biology. 1971 ; Vol. 60, No. 1. pp. 113-122.
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