Complex of aminoacyl-transfer RNA synthetases

Alok Kumar Bandyopadhyay, Murray P. Deutscher

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143 Scopus citations

Abstract

A high molecular weight complex has been isolated from rat liver which contains all the aminoacyl-transfer RNA synthetases and and a large proportion of the tRNA present in the high-speed supernatant fraction. The complex could be partially purified by chromatography on Sephadex G200 and Sepharose 6B. The complex could be disrupted by excess homogenization and freezing and thawing with a concomitant decrease in the activities of the aminoacyl-tRNA synthetases. The results suggest that some of the presumed "soluble" components of the protein synthetic apparatus may be present in highly organized structures within the cell.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalJournal of molecular biology
Volume60
Issue number1
DOIs
StatePublished - Aug 28 1971

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ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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