Complex of aminoacyl-transfer RNA synthetases

Alok Kumar Bandyopadhyay; Murray P. Deutscher

doi:10.1016/0022-2836(71)90451-7

Complex of aminoacyl-transfer RNA synthetases

Alok Kumar Bandyopadhyay, Murray P. Deutscher

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article

143 Scopus citations

Abstract

A high molecular weight complex has been isolated from rat liver which contains all the aminoacyl-transfer RNA synthetases and and a large proportion of the tRNA present in the high-speed supernatant fraction. The complex could be partially purified by chromatography on Sephadex G200 and Sepharose 6B. The complex could be disrupted by excess homogenization and freezing and thawing with a concomitant decrease in the activities of the aminoacyl-tRNA synthetases. The results suggest that some of the presumed "soluble" components of the protein synthetic apparatus may be present in highly organized structures within the cell.

Original language	English (US)
Pages (from-to)	113-122
Number of pages	10
Journal	Journal of molecular biology
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(71)90451-7
State	Published - Aug 28 1971

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ASJC Scopus subject areas

Structural Biology
Molecular Biology

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Bandyopadhyay, A. K., & Deutscher, M. P. (1971). Complex of aminoacyl-transfer RNA synthetases. Journal of molecular biology, 60(1), 113-122. https://doi.org/10.1016/0022-2836(71)90451-7

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10.1016/0022-2836(71)90451-7