Complex formation between chlorophyll a and cytochrome c: surface properties at the air‐water interface: Absorbance, fluorescence and fluorescence‐lifetime in Langmuir‐Blodgett films

François LAMARCHE, Gilles PICARD, Françoise TÉCHY, Jacques AGHION, Roger M. LEBLANC

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The binding of cytochrome c to an insoluble monolayer of chlorophyll a was studied. Surface pressure (II), surface potential (Δ V) and [14C]cytochrome c surface-concentration (Γ) isotherms were measured versus molecular area (σ) in mixed films. Compared to the successive-addition method, this procedure allows the formation of homogeneous mixed films. The cytochrome c is incorporated into a ophyll a monolayer, compressed at a surface pressure of 20 mN · m-1. On expansion, the quantity of protein incorporated into the monolayer gradually increases. Subsequent compression-expansion cycles result in similar isotherms, distinct from that measured during the first expansion. All surface properties measured, but more specifically the surface radioactivity of [14C]cytochrome c, indicate the irreversibility of protein incorporation into the chlorophyll a monolayer. In fact, surface properties of the binary film are completely different from the properties of either of the pure components. As a result, calculated values of surface potentials for mixed films using the additivity law deviate from experimentally measured potentials. The absorption and fluorescence spectra of mixed films transferred onto a solid substrate by the Langmuir-Blodgett technique, indicate a dilution effect of chlorophyll a by cytochrome c. However, the dilution effect cannot be detected by the fluorescence lifetimes of pure chlorophyll a and mixed chlorophyll a-cytochrome c films, both shorter than 0.2 ns. This provides support for the existence of an energytransfer mechanism between chlorophyll a monomer and chlorophyll a aggregates which could serve as an energy trap. The role of the protein could be related to that of the matrix.

Original languageEnglish (US)
Pages (from-to)529-534
Number of pages6
JournalEuropean Journal of Biochemistry
Volume197
Issue number2
DOIs
StatePublished - Apr 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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