Complex determinants of macrophage tropism in env of simian immunodeficiency virus

K. Mori, D. J. Ringler, T. Kodama, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Macrophage-tropic virus variants evolved during the course of infection of individual rhesus monkeys with cloned, non-macrophagetropic simian immunodeficiency virus. Specific changes in the envelope gene (env) were found to be primarily responsible for the dramatic increase in the ability of the virus to replicate in macrophages. Cloned viruses differing at nine amino acid positions in env exhibited a more than 100-fold difference in replicative capacity for primary cultures of rhesus monkey alveolar macrophages. At least five of the nine amino acid changes contributed to macrophage tropism. These determinants were distributed across the full length of env, including both the gp120 and gp41 products of the env gene. Furthermore, the emergence of macrophagetropic variants in vivo was associated with specific pathologic manifestations in which the macrophage is the major infected cell type. Thus, major determinants of macrophage tropism reside in env, they can be complex in nature, and the presence of macrophage- tropic virus variants in vivo can influence the disease course and disease manifestations.

Original languageEnglish (US)
Pages (from-to)2067-2075
Number of pages9
JournalJournal of Virology
Volume66
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
tropisms
Tropism
macrophages
Macrophages
Viruses
viruses
Macaca mulatta
tropics
env Gene Products
env Genes
Amino Acids
Alveolar Macrophages
amino acids
disease course
genes
Infection
infection

ASJC Scopus subject areas

  • Immunology

Cite this

Complex determinants of macrophage tropism in env of simian immunodeficiency virus. / Mori, K.; Ringler, D. J.; Kodama, T.; Desrosiers, Ronald Charles.

In: Journal of Virology, Vol. 66, No. 4, 1992, p. 2067-2075.

Research output: Contribution to journalArticle

Mori, K. ; Ringler, D. J. ; Kodama, T. ; Desrosiers, Ronald Charles. / Complex determinants of macrophage tropism in env of simian immunodeficiency virus. In: Journal of Virology. 1992 ; Vol. 66, No. 4. pp. 2067-2075.
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