Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16

P. Huhtala, R. L. Eddy, Yao-Shan Fan, M. G. Byers, T. B. Shows, K. Tryggvason

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The complete amino acid sequence of the human type IV collagenase preproenzyme was determined from cDNA and genomic clones. Primer extension and S1 nuclease analyses as well as nucleotide sequencing of a genomic clone indicate that the first exon has two closely spaced initiation sites for transcription and codes for 290 and 280 nt of a 5′ untranslated region and a 29-residue signal peptide. The gene (CLG4) was localized to 16q21 using somatic cell hybrids and in situ hybridization.

Original languageEnglish
Pages (from-to)554-559
Number of pages6
JournalGenomics
Volume6
Issue number3
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

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Chromosomes, Human, Pair 16
Collagenases
Clone Cells
Hybrid Cells
5' Untranslated Regions
Transcription Initiation Site
Protein Sorting Signals
Genes
In Situ Hybridization
Amino Acid Sequence
Exons
Nucleotides
Complementary DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16. / Huhtala, P.; Eddy, R. L.; Fan, Yao-Shan; Byers, M. G.; Shows, T. B.; Tryggvason, K.

In: Genomics, Vol. 6, No. 3, 01.01.1990, p. 554-559.

Research output: Contribution to journalArticle

Huhtala, P. ; Eddy, R. L. ; Fan, Yao-Shan ; Byers, M. G. ; Shows, T. B. ; Tryggvason, K. / Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16. In: Genomics. 1990 ; Vol. 6, No. 3. pp. 554-559.
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