Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16

P. Huhtala, R. L. Eddy, Y. S. Fan, M. G. Byers, T. B. Shows, K. Tryggvason

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The complete amino acid sequence of the human type IV collagenase preproenzyme was determined from cDNA and genomic clones. Primer extension and S1 nuclease analyses as well as nucleotide sequencing of a genomic clone indicate that the first exon has two closely spaced initiation sites for transcription and codes for 290 and 280 nt of a 5′ untranslated region and a 29-residue signal peptide. The gene (CLG4) was localized to 16q21 using somatic cell hybrids and in situ hybridization.

Original languageEnglish (US)
Pages (from-to)554-559
Number of pages6
JournalGenomics
Volume6
Issue number3
DOIs
StatePublished - Mar 1990
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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