Complete nucleotide sequence of htlv-ii isolate nra: Comparison of envelope sequence variation of htlv-ii isolates from u.s. Blood donors and u.s. and italian iv drug users

Helen Lee, Kenneth B. Idler, Priscilla Swanson, Jose J. Aparicio, Kenneth K. Chin, Jerome P. Lax, Minh Nguyen, Terri Mann, Gregor Leckie, Alessandro Zanetti, Giovanni Marinucci, Irvin S.Y. Chen, Joseph D. Rosenblatt

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The entire nucleotide sequence of human T-cell lymphotropic virus type II (HTLV-II) from a previously described isolate of patient NRA (HTLV-II(NRA)) was determined. Clones encoding the 5' LTR and gag, pol, env and tax/rex open reading frames were subcloned and sequenced on both strands. The provirus consisted of 8957 nucleotides and showed 95.2% homology with the HTLV-II(Mo) prototype at the nucleotide level. Less than 5% amino acid variation between HTLV-II(NRA) and HTLV-II(Mo) was observed for coding regions. Although isolate HTLV-II(NRA) had an additional 25 amino acids at the 3' end of tax/rex, this region was 96% homologous with the 5' end of HTLV-II(Mo) 3' LTR. To further investigate HTLV-II variability, a portion of the env gp46 gene derived from 9 HTLV-II infected persons was amplified by polymerase chain reaction and sequenced. Sequence was obtained for 320 nucleotides corresponding to HTLV-II(Mo) positions 5291 to 5610. Isolates similar to the HTLV-II(Mo) and HTLV-II(NRA) prototypes were identified, and sequences were highly conserved.

Original languageEnglish (US)
Pages (from-to)57-69
Number of pages13
JournalVirology
Volume196
Issue number1
DOIs
StatePublished - Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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