Complementary DNA cloning of a μ-opioid receptor from rat pertitoneal macrophages

M. Sedqi, Sabita Roy, Sundaram Ramakrishnan, R. Elde, H. H. Loh

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101 Citations (Scopus)

Abstract

Treatment with opioid agonists in vitro and in vivo has been shown to affect the function of the immune system. Several investigators have suggested that immune cells may express opioid receptors, but it had been very difficult to demonstrate their presence on these cells by direct binding assays. Our earlier studies have shown that macrophage progenitor cells are highly sensitive to morphine treatment in vitro and in vivo. In the current investigation, we determined, unequivocally, the expression of μ-opioid receptor related transcripts in rat peritoneal macrophages by reverse transcriptase-polymerase chain reaction (RT-PCR) studies. In order to further characterize the transcript, the RT-PCR product was cloned and sequenced. The sequence analyses indicate that the transcripts from rat peritoneal macrophages are identical to those for the μ-opioid receptor described in the rat brain. To further confirm the presence of μ-opioid receptors, immunoreactivity to an antiserum raised against the carboxyl terminal fifteen amino acid residues of the μ-opioid receptor was determined. These studies show for the first time that rat peritoneal macrophages express a μ-opioid receptor.

Original languageEnglish (US)
Pages (from-to)563-574
Number of pages12
JournalBiochemical and Biophysical Research Communications
Volume209
Issue number2
DOIs
StatePublished - Apr 17 1995
Externally publishedYes

Fingerprint

Macrophages
Cloning
Opioid Receptors
Rats
Organism Cloning
Complementary DNA
Peritoneal Macrophages
Polymerase chain reaction
RNA-Directed DNA Polymerase
Reverse Transcriptase Polymerase Chain Reaction
Immune system
Reaction products
Morphine
Opioid Analgesics
Sequence Analysis
Immune Sera
Immune System
Assays
Brain
Stem Cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Complementary DNA cloning of a μ-opioid receptor from rat pertitoneal macrophages. / Sedqi, M.; Roy, Sabita; Ramakrishnan, Sundaram; Elde, R.; Loh, H. H.

In: Biochemical and Biophysical Research Communications, Vol. 209, No. 2, 17.04.1995, p. 563-574.

Research output: Contribution to journalArticle

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