Competitive membrane adsorption of Na+, K+, and Ca2+ in smooth muscle cells

Michael A Kolber, Cornelis van Breemen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalThe Journal of Membrane Biology
Volume58
Issue number2
DOIs
StatePublished - Jun 1 1981

Fingerprint

Adsorption
Smooth Muscle Myocytes
Monovalent Cations
Membranes
Myofibrils
Divalent Cations
Smooth Muscle
Guinea Pigs
Colon
Pharmacology

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Competitive membrane adsorption of Na+, K+, and Ca2+ in smooth muscle cells. / Kolber, Michael A; van Breemen, Cornelis.

In: The Journal of Membrane Biology, Vol. 58, No. 2, 01.06.1981, p. 115-121.

Research output: Contribution to journalArticle

@article{242c298249c047069a5144e4f0016a9e,
title = "Competitive membrane adsorption of Na+, K+, and Ca2+ in smooth muscle cells",
abstract = "A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.",
author = "Kolber, {Michael A} and {van Breemen}, Cornelis",
year = "1981",
month = "6",
day = "1",
doi = "10.1007/BF01870974",
language = "English",
volume = "58",
pages = "115--121",
journal = "Journal of Membrane Biology",
issn = "0022-2631",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Competitive membrane adsorption of Na+, K+, and Ca2+ in smooth muscle cells

AU - Kolber, Michael A

AU - van Breemen, Cornelis

PY - 1981/6/1

Y1 - 1981/6/1

N2 - A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.

AB - A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.

UR - http://www.scopus.com/inward/record.url?scp=0019510695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019510695&partnerID=8YFLogxK

U2 - 10.1007/BF01870974

DO - 10.1007/BF01870974

M3 - Article

VL - 58

SP - 115

EP - 121

JO - Journal of Membrane Biology

JF - Journal of Membrane Biology

SN - 0022-2631

IS - 2

ER -