Abstract
A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.
| Original language | English |
|---|---|
| Pages (from-to) | 115-121 |
| Number of pages | 7 |
| Journal | The Journal of Membrane Biology |
| Volume | 58 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 1 1981 |
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ASJC Scopus subject areas
- Physiology
- Cell Biology
- Biophysics
Cite this
Competitive membrane adsorption of Na+, K+, and Ca2+ in smooth muscle cells. / Kolber, Michael A; van Breemen, Cornelis.
In: The Journal of Membrane Biology, Vol. 58, No. 2, 01.06.1981, p. 115-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Competitive membrane adsorption of Na+, K+, and Ca2+ in smooth muscle cells
AU - Kolber, Michael A
AU - van Breemen, Cornelis
PY - 1981/6/1
Y1 - 1981/6/1
N2 - A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.
AB - A theory for Na+, K+ and Ca2+ competitive adsorption to a charged membrane is used to explain a number of experimental observations in smooth muscle. Adsorption is described by Langmuir isotherms for mono- and divalent cations which in turn are coupled in a self-consistent way to the bulk solution through the diffuse double layer theory and the Boltzman equations. We found that the dissociation constants for binding of Na+, K+ and Ca2+ in guinea pig taenia coli are ca. 0.009, 1.0, and 4×10-8m, respectively. Furthermore, the effect of a Ca2+ pump that maintains free surface Ca2+ concentration constant is investigated. A decrease in intracellular Na+ content results in an increased Ca2+ uptake; part of this uptake is due to an increase in surface-bound Ca2+ in an intracellular compartment which is in contact with the myofilaments. Variations in the amount of charge available to bind Ca2+ and the surface charge density are studied and their effect interpreted in terms of different pharmacological agents.
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U2 - 10.1007/BF01870974
DO - 10.1007/BF01870974
M3 - Article
VL - 58
SP - 115
EP - 121
JO - Journal of Membrane Biology
JF - Journal of Membrane Biology
SN - 0022-2631
IS - 2
ER -