Comparison of the effects of ammonia on brain mitochondrial function in rats and gulf toadfish

Clémence M. Veauvy, Yuxiang Wang, Patrick J. Walsh, Miguel Perez-Pinzon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We compared the effect of hyperammonemia on NADH levels in brain slices and on the rate of oxygen consumption from isolated nonsynaptic brain mitochondria in ammonia-sensitive Wistar rats with that in ammonia-tolerant gulf toadfish (Opsanus beta). The NADH content was significantly decreased (12% less than control after 45 min with 1 mM NH4Cl) in rat brain slices, but it was not affected in brain slices from toadfish (with both 1 and 6 mM NH4Cl). The rates of oxygen consumption of different sets of enzymes of the electron transport chain (ETC; complexes I, II, III, and IV; II, III, and IV; and IV alone) were unaltered by hyperammonemic conditions in isolated nonsynaptic mitochondria from either rats or toadfish. These results lead us to conclude that the differing effects of ammonia on NADH levels in rat and toadfish brain slices must be due to aspects other than the direct effects of ammonia on enzymes of the ETC. Additionally, because these effects were seen in vitro, our studies enabled us to rule out the possibility that effects of ammonia on metabolism were via indirect systemic effects. These results are discussed in the context of current views on mechanisms of central nervous system damage in hyperammonemic states.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume283
Issue number3 52-3
StatePublished - Sep 1 2002

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Batrachoidiformes
Ammonia
NAD
Brain
Oxygen Consumption
Mitochondria
Hyperammonemia
Enzymes
Electron Transport
Wistar Rats
Central Nervous System

Keywords

  • Glutamine metabolism
  • Hepatic encephalopathy
  • Hyperammonemia
  • Mitochondria
  • NADH
  • Opsanus beta

ASJC Scopus subject areas

  • Physiology

Cite this

Comparison of the effects of ammonia on brain mitochondrial function in rats and gulf toadfish. / Veauvy, Clémence M.; Wang, Yuxiang; Walsh, Patrick J.; Perez-Pinzon, Miguel.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 283, No. 3 52-3, 01.09.2002.

Research output: Contribution to journalArticle

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