Comparison of Skin Antiseptic Agents and the Role of 0.01% Hypochlorous Acid

Ann Q. Tran, Nicole Topilow, Andrew Rong, Patrice J. Persad, Michael C. Lee, James H. Lee, Apostolos G. Anagnostopoulos, Wendy W. Lee

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Background: Hypochlorous acid (HA) has both anti-microbial and wound-healing properties with a growing role for utilization in pre-procedural care on the face. Objectives: The authors sought to compare the antiseptic property of 0.01% HA solution, 5% povidone iodine (PI), 4% chlorhexidine gluconate (CHG), and 70% isopropyl alcohol (IPA) antiseptic on facial skin. Methods: This was a prospective single-center clinical trial. Results: A total of 21 participants were recruited. Bacterial growth was seen in CHG (10%), IPA (71%), PI (81%), and HA (95%) of specimens (P < 0.001). CHG had less growth compared with HA (P = <0.001), IPA (P = <0.001), and PI (P = <0.001). No difference in bacterial growth was noted between HA and IPA (P = 0.063) or HA and PI (P = 0.25). Significant differences in mono-microbial and poly-microbial growth were seen between HA and IPA (P = 0.046) and HA and CHG (P = <0.001). Staphylococcus epidermidis grew less frequently in CHG (10%), followed by IPA (29%), PI (71%), and HA (71%). Staphylococcus capitis grew less frequently in CHG (0%), followed by PI (14%), HA (24%), and IPA (29%). Conclusions: CHG reduced the bacterial growth compared with HA, PI, and IPA. However, HA, PI, and IPA had insignificant differences in bactericidal effects. Our study provides a supporting role of HA to be considered as an antiseptic. Level of Evidence: 2:.

Original languageEnglish (US)
Pages (from-to)1170-1175
Number of pages6
JournalAesthetic Surgery Journal
Issue number10
StatePublished - Oct 1 2021

ASJC Scopus subject areas

  • Surgery


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