Comparison of packing material in an animal model of middle ear trauma

Enrique Perez, Ralph Abi Hachem, Daniel Carlton, Isabel Bueno, Stephen Vernon, Thomas R. Van De Water, Simon I. Angeli

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Purpose To compare the performance of absorbable gelatin sponge (AGS) with polyurethane foam (PUF) as middle ear packing material after mucosal trauma. Materials and methods Using a randomized, controlled and blinded study design fifteen guinea pigs underwent middle ear surgery with mucosal trauma performed on both ears. One ear was packed with either PUF or AGS while the contralateral ear remained untreated and used as non-packed paired controls. Auditory brainstem response (ABR) thresholds were measured pre-operatively and repeated at 1, 2, and 6 weeks postoperatively. Histological analysis of middle ear mucosa was done in each group to evaluate the inflammatory reaction and wound healing. Another eighteen animals underwent middle ear wounding and packing in one ear while the contralateral ear was left undisturbed as control. Twelve guinea pigs were euthanized at 2 weeks postoperatively, and six were euthanized at 3 days post-operatively. Mucosal samples were collected for analysis of TGF-β1 levels by enzyme-linked immunosorbent assay. Results ABR recordings demonstrate that threshold level changes from baseline were minor in PUF packed and control ears. Threshold levels were higher in the AGS packed ears compared with both control and PUF packed ears for low frequency stimuli. Histological analysis showed persistence of packing material at 6 weeks postoperatively, inflammation, granulation tissue formation, foreign body reaction and neo-osteogenesis in both AGS and PUF groups. TGF-β1 protein levels did not differ between groups. Conclusion PUF and AGS packing cause inflammation and neo-osteogenesis in the middle ear following wounding of the mucosa and packing.

Original languageEnglish (US)
Pages (from-to)323-329
Number of pages7
JournalAmerican Journal of Otolaryngology - Head and Neck Medicine and Surgery
Issue number4
StatePublished - Jul 1 2016

ASJC Scopus subject areas

  • Otorhinolaryngology


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