Comparison of NAA measures by MRS and HPLC.

P. P. Fatouros, D. L. Heath, A. Beaumont, F. D. Corwin, S. Signoretti, R. H. al-Samsam, B. Alessandri, P. Lazzarino, R. Vagnozzi, B. Tavazzi, Ross Bullock, A. Marmarou

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work investigates the accuracy of an in vivo estimation of absolute N-acetyl aspartate (NAA) concentrations by magnetic resonance spectroscopy (MRS) using cerebral water as an internal reference standard. Single-voxel, proton spectroscopy was carried out in two groups of rats (normal and diffuse head injury), using a PRESS sequence with TR = 3 s, TE = 135 ms. Fully relaxed water spectra and water-suppressed proton spectra were obtained from a 7 x 5 x 5 mm3 volume of tissue. MRI-based brain water content measurements were also performed. Following MRS, HPLC determinations of NAA were carried out. In the normal rats the MRS yielded 10.98 +/- 0.83 mmol/kg w.w. vs 10.76 +/- 0.76 for HPLC with a mean absolute difference of 0.8. In the injured rats the corresponding results were 9.41 +/- 1.78 (MRS) and 8.16 +/- 0.77 (HPLC) with a mean absolute difference of 1.66. The in vivo absolute method accurately documented the temporal NAA changes compared to the NAA/Cr approach.

Original languageEnglish
Pages (from-to)35-37
Number of pages3
JournalActa Neurochirurgica, Supplement
Volume76
StatePublished - Dec 1 2000
Externally publishedYes

Fingerprint

Magnetic resonance spectroscopy
aspartates
magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
rats
Rats
Water
Protons
water
protons
Craniocerebral Trauma
Magnetic resonance imaging
Water content
moisture content
brain
Brain
Spectrum Analysis
Spectroscopy
Tissue

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology

Cite this

Fatouros, P. P., Heath, D. L., Beaumont, A., Corwin, F. D., Signoretti, S., al-Samsam, R. H., ... Marmarou, A. (2000). Comparison of NAA measures by MRS and HPLC. Acta Neurochirurgica, Supplement, 76, 35-37.

Comparison of NAA measures by MRS and HPLC. / Fatouros, P. P.; Heath, D. L.; Beaumont, A.; Corwin, F. D.; Signoretti, S.; al-Samsam, R. H.; Alessandri, B.; Lazzarino, P.; Vagnozzi, R.; Tavazzi, B.; Bullock, Ross; Marmarou, A.

In: Acta Neurochirurgica, Supplement, Vol. 76, 01.12.2000, p. 35-37.

Research output: Contribution to journalArticle

Fatouros, PP, Heath, DL, Beaumont, A, Corwin, FD, Signoretti, S, al-Samsam, RH, Alessandri, B, Lazzarino, P, Vagnozzi, R, Tavazzi, B, Bullock, R & Marmarou, A 2000, 'Comparison of NAA measures by MRS and HPLC.', Acta Neurochirurgica, Supplement, vol. 76, pp. 35-37.
Fatouros PP, Heath DL, Beaumont A, Corwin FD, Signoretti S, al-Samsam RH et al. Comparison of NAA measures by MRS and HPLC. Acta Neurochirurgica, Supplement. 2000 Dec 1;76:35-37.
Fatouros, P. P. ; Heath, D. L. ; Beaumont, A. ; Corwin, F. D. ; Signoretti, S. ; al-Samsam, R. H. ; Alessandri, B. ; Lazzarino, P. ; Vagnozzi, R. ; Tavazzi, B. ; Bullock, Ross ; Marmarou, A. / Comparison of NAA measures by MRS and HPLC. In: Acta Neurochirurgica, Supplement. 2000 ; Vol. 76. pp. 35-37.
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