Comparison of NAA measures by MRS and HPLC.

P. P. Fatouros, D. L. Heath, A. Beaumont, F. D. Corwin, S. Signoretti, R. H. al-Samsam, B. Alessandri, P. Lazzarino, R. Vagnozzi, B. Tavazzi, R. Bullock, A. Marmarou

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5 Scopus citations


This work investigates the accuracy of an in vivo estimation of absolute N-acetyl aspartate (NAA) concentrations by magnetic resonance spectroscopy (MRS) using cerebral water as an internal reference standard. Single-voxel, proton spectroscopy was carried out in two groups of rats (normal and diffuse head injury), using a PRESS sequence with TR = 3 s, TE = 135 ms. Fully relaxed water spectra and water-suppressed proton spectra were obtained from a 7 x 5 x 5 mm3 volume of tissue. MRI-based brain water content measurements were also performed. Following MRS, HPLC determinations of NAA were carried out. In the normal rats the MRS yielded 10.98 +/- 0.83 mmol/kg w.w. vs 10.76 +/- 0.76 for HPLC with a mean absolute difference of 0.8. In the injured rats the corresponding results were 9.41 +/- 1.78 (MRS) and 8.16 +/- 0.77 (HPLC) with a mean absolute difference of 1.66. The in vivo absolute method accurately documented the temporal NAA changes compared to the NAA/Cr approach.

Original languageEnglish (US)
Pages (from-to)35-37
Number of pages3
JournalActa neurochirurgica. Supplement
StatePublished - 2000

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology


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