Comparison of dopamine uptake and release in vitro in sheep and rat striatum

H. M. Jacocks, S. Izenwasser, L. L. Werling, B. M. Cox

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Cocaine inhibits tritium-labeled dopamine ([3H]DA) uptake in rat (IC50≈400 nM) and sheep (IC50≈1 μM) striatum. GBR 12909, a selective DA uptake inhibitor, potently inhibits [3H]DA uptake in rat (IC50<10 nM), but is less effective (only 60% of the uptake is inhibited at a concentration of 10 μM) and less potent (IC50≈300 nM) in sheep. [3H]DA release from slices of rat or sheep striatum is stimulated by potassium (15-50 mM). In the presence of nomifensine (10 μM), cocaine (10 μM) had no effect on potassium-stimulated [3H]DA release in either species. [3H]DA release in increased by N-methyl-D-aspartate (NMDA) (10-1000 μM) in rat striatum but NMDA did not stimulate [3H]DA release in sheep striatum. These findings suggest that NMDA receptors either are absent from or do not regulate release of preloaded [3H]DA in sheep striatum.

Original languageEnglish (US)
Pages (from-to)481-488
Number of pages8
JournalLife Sciences
Volume49
Issue number7
DOIs
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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