Abstract
To get insights into the principles of gene expression changes during cardiac hypertrophy, three rat cardiac hypertrophy models were prepared, i.e., suprarenal abdominal aortic stenosis (SRS), arterial-vein fistula (AVF) and continuous jugular vein infusion of norepinephrine (NEi). The cardiac function and structure were analyzed by echocardiograph as well as histological examination. Total RNA of left ventricles was extracted and gene expression profiles were analyzed by cDNA microarray. SRS and NEi induced concentric cardiac hypertrophy and AVF induced eccentric hypertrophy in rats, among which NEi caused obvious cardiac fibrosis. The changes of gene expression profiles were compared comprehensively across different pathologic cardiac hypertrophy models. While gene expression profiles of different cardiac hypertrophy models compared with pairs, parts of the genes involved were found overlapped, and mostly the gene expression changed in the same direction between two models, but some of them changed in the opposite directions. Expression levels of 19 genes were found changed across all cardiac hypertrophy models, and genes relatively regulated in a specific model was also found when comparison of all the three models was carried out. Novel clues for further study might derive from the results mentioned above, and some genes might be the marker genes of cardiac hypertrophy or the targets of therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 210-218 |
| Number of pages | 9 |
| Journal | Sheng li xue bao [Acta physiologica Sinica] |
| Volume | 56 |
| Issue number | 2 |
| State | Published - Apr 25 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physiology
Cite this
Comparison of changes in left ventricular gene expression profiles from different cardiac hypertrophy models in rats. / Li, Ping; Li, Jin Liang; Feng, Xin Heng; Li, Zhao Ping; Yin, Feng; Yan, Jie; Hou, Rong; Han, Qi De; Zhang, You Yi.
In: Sheng li xue bao [Acta physiologica Sinica], Vol. 56, No. 2, 25.04.2004, p. 210-218.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of changes in left ventricular gene expression profiles from different cardiac hypertrophy models in rats
AU - Li, Ping
AU - Li, Jin Liang
AU - Feng, Xin Heng
AU - Li, Zhao Ping
AU - Yin, Feng
AU - Yan, Jie
AU - Hou, Rong
AU - Han, Qi De
AU - Zhang, You Yi
PY - 2004/4/25
Y1 - 2004/4/25
N2 - To get insights into the principles of gene expression changes during cardiac hypertrophy, three rat cardiac hypertrophy models were prepared, i.e., suprarenal abdominal aortic stenosis (SRS), arterial-vein fistula (AVF) and continuous jugular vein infusion of norepinephrine (NEi). The cardiac function and structure were analyzed by echocardiograph as well as histological examination. Total RNA of left ventricles was extracted and gene expression profiles were analyzed by cDNA microarray. SRS and NEi induced concentric cardiac hypertrophy and AVF induced eccentric hypertrophy in rats, among which NEi caused obvious cardiac fibrosis. The changes of gene expression profiles were compared comprehensively across different pathologic cardiac hypertrophy models. While gene expression profiles of different cardiac hypertrophy models compared with pairs, parts of the genes involved were found overlapped, and mostly the gene expression changed in the same direction between two models, but some of them changed in the opposite directions. Expression levels of 19 genes were found changed across all cardiac hypertrophy models, and genes relatively regulated in a specific model was also found when comparison of all the three models was carried out. Novel clues for further study might derive from the results mentioned above, and some genes might be the marker genes of cardiac hypertrophy or the targets of therapy.
AB - To get insights into the principles of gene expression changes during cardiac hypertrophy, three rat cardiac hypertrophy models were prepared, i.e., suprarenal abdominal aortic stenosis (SRS), arterial-vein fistula (AVF) and continuous jugular vein infusion of norepinephrine (NEi). The cardiac function and structure were analyzed by echocardiograph as well as histological examination. Total RNA of left ventricles was extracted and gene expression profiles were analyzed by cDNA microarray. SRS and NEi induced concentric cardiac hypertrophy and AVF induced eccentric hypertrophy in rats, among which NEi caused obvious cardiac fibrosis. The changes of gene expression profiles were compared comprehensively across different pathologic cardiac hypertrophy models. While gene expression profiles of different cardiac hypertrophy models compared with pairs, parts of the genes involved were found overlapped, and mostly the gene expression changed in the same direction between two models, but some of them changed in the opposite directions. Expression levels of 19 genes were found changed across all cardiac hypertrophy models, and genes relatively regulated in a specific model was also found when comparison of all the three models was carried out. Novel clues for further study might derive from the results mentioned above, and some genes might be the marker genes of cardiac hypertrophy or the targets of therapy.
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M3 - Article
VL - 56
SP - 210
EP - 218
JO - Sheng li xue bao [Acta physiologica Sinica]
JF - Sheng li xue bao [Acta physiologica Sinica]
SN - 0371-0874
IS - 2
ER -