Comparative study of different seeding methods based on a multilayer SIS scaffold: Which is the optimal procedure for urethral tissue engineering?

Xiang Guo Lv, Chao Feng, Qiang Fu, Hong Xie, Ying Wang, Jian Wen Huang, Min Kai Xie, Anthony Atala, Yue Min Xu, Wei Xin Zhao

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Seeding cells efficiently and uniformly onto three-dimensional scaffolds is key for engineering urological tissue with an ideal histological structure in vitro. Using an optimized seeding technology allows cells to cooperate positively with biomaterials, resulting in successful reconstructive surgery. In this study, we used four different types of seeding methods in a scaffold of small intestinal submucosa (SIS). The efficiency of the sandwich co-culture, layered co-culture, static-agitation seeding, and centrifugation seeding methods were compared. It was demonstrated that dynamic seeding methods, such as static-agitation and centrifugation seeding, had superior cell-matrix infiltration and mechanical properties. The seeding time could be reduced by 5-10 min using the centrifugation method. Furthermore, functional assessment of the barriers revealed that this function was better in the centrifugation seeding method than in any other method. Our study suggests that both the static-agitation and centrifugation methods are suitable for cell seeding on SIS. There is no significant change in surface area of SIS with different seeding methods. These methods reinforce the physiological and mechanical properties of biomaterials and allow for the future in vivo study of tissue-engineered urethral reconstruction.

Original languageEnglish (US)
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
StateAccepted/In press - 2015
Externally publishedYes

Keywords

  • Centrifugation seeding
  • Seeding technology
  • Static-agitation seeding
  • Urethra reconstruction

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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