Comparative studies on urokinase and plasminogen activator from cultured pancreatic carcinoma

Ming Chi Wu; Adel A. Yunis

doi:10.1016/0020-711X(79)90080-6

Comparative studies on urokinase and plasminogen activator from cultured pancreatic carcinoma

Ming Chi Wu, Adel A. Yunis

Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

1. 1. Plasminogen activator (PA) from a human pancreatic carcinoma cell line and human urinary urokinase (UK) have been purified to homogeneity. Amino acid analysis indicated they have a similar amino acid composition. 2. 2. Antibodies against both PA and UK were prepared in rabbits. Immunodiffusion experiments revealed that both enzymes were immunologically identical. 3. 3. Antibodies against PA and UK cross inhibited PAs from several other human cancer cell lines, but did not inhibit PA from cultured rat breast carcinoma R2426 cells.

Original language	English (US)
Pages (from-to)	1001-1006
Number of pages	6
Journal	International Journal of Biochemistry
Volume	10
Issue number	12
DOIs	https://doi.org/10.1016/0020-711X(79)90080-6
State	Published - 1979

ASJC Scopus subject areas

Biochemistry

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10.1016/0020-711X(79)90080-6

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title = "Comparative studies on urokinase and plasminogen activator from cultured pancreatic carcinoma",

abstract = "1. 1. Plasminogen activator (PA) from a human pancreatic carcinoma cell line and human urinary urokinase (UK) have been purified to homogeneity. Amino acid analysis indicated they have a similar amino acid composition. 2. 2. Antibodies against both PA and UK were prepared in rabbits. Immunodiffusion experiments revealed that both enzymes were immunologically identical. 3. 3. Antibodies against PA and UK cross inhibited PAs from several other human cancer cell lines, but did not inhibit PA from cultured rat breast carcinoma R2426 cells.",

author = "Wu, {Ming Chi} and Yunis, {Adel A.}",

note = "Funding Information: Acknowledgements-Thea uthors wish to thank Dr Astedto f the Universityo f Lund, Swedenf, or providingu s the anti-UK II antibody,t o Mr C. P. Hsieh of Taiwan of TaitungC o. for his gift of partialp urifiedu rokinasea, nd to Dr Keith Brew,D ept of BiochemistryU, niversityo f Miami Schoolo f Medicine,f or the amino acid compositiona naly-sis. This work was supportedi n part by USPH grants CA 19182a nd by grant No. IN-51s from the American Cancer Society.D r Adel A. Yunis is a Howard Hughes Investigator.",

year = "1979",

doi = "10.1016/0020-711X(79)90080-6",

language = "English (US)",

volume = "10",

pages = "1001--1006",

journal = "International Journal of Biochemistry",

issn = "1357-2725",

publisher = "Elsevier Limited",

number = "12",

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T1 - Comparative studies on urokinase and plasminogen activator from cultured pancreatic carcinoma

AU - Wu, Ming Chi

AU - Yunis, Adel A.

N1 - Funding Information: Acknowledgements-Thea uthors wish to thank Dr Astedto f the Universityo f Lund, Swedenf, or providingu s the anti-UK II antibody,t o Mr C. P. Hsieh of Taiwan of TaitungC o. for his gift of partialp urifiedu rokinasea, nd to Dr Keith Brew,D ept of BiochemistryU, niversityo f Miami Schoolo f Medicine,f or the amino acid compositiona naly-sis. This work was supportedi n part by USPH grants CA 19182a nd by grant No. IN-51s from the American Cancer Society.D r Adel A. Yunis is a Howard Hughes Investigator.

PY - 1979

Y1 - 1979

N2 - 1. 1. Plasminogen activator (PA) from a human pancreatic carcinoma cell line and human urinary urokinase (UK) have been purified to homogeneity. Amino acid analysis indicated they have a similar amino acid composition. 2. 2. Antibodies against both PA and UK were prepared in rabbits. Immunodiffusion experiments revealed that both enzymes were immunologically identical. 3. 3. Antibodies against PA and UK cross inhibited PAs from several other human cancer cell lines, but did not inhibit PA from cultured rat breast carcinoma R2426 cells.

AB - 1. 1. Plasminogen activator (PA) from a human pancreatic carcinoma cell line and human urinary urokinase (UK) have been purified to homogeneity. Amino acid analysis indicated they have a similar amino acid composition. 2. 2. Antibodies against both PA and UK were prepared in rabbits. Immunodiffusion experiments revealed that both enzymes were immunologically identical. 3. 3. Antibodies against PA and UK cross inhibited PAs from several other human cancer cell lines, but did not inhibit PA from cultured rat breast carcinoma R2426 cells.

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Comparative studies on urokinase and plasminogen activator from cultured pancreatic carcinoma

Abstract

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