Comparative photodynamic effect of Rose Bengal, Erythrosin B and dihematoporphyrin ethers on lens epithelial cells

Yoshiko Takesue, Mei Mei Mui, Takahiko Hachiya, Jean Marie Parel

Research output: Contribution to journalConference articlepeer-review


The proliferation of residual lens epithelial cells (LEC) is responsible for opacification of the lens capsule and loss of visual acuity after cataract surgery. Photodynamic therapy (PDT) using Di-Hematoporphyrin Ether (DHE) was shown to kid cultured LEC. In this study, we studied 2 synthetic photodynamic drugs: Rose Bengal (RB) and Erythrosin B (EB) to determine the minimal PDT parameters. LEC located on the anterior capsule of freshly explanted rabbit lenses were subcultured for the following studies: (1) Dye uptake was evaluated by cellular fluorescence. The minimum concentrations for RB and EB after 1 minute of exposure were 1 μg/ml, and 50 μg/ml; (2) Cytotoxicity was evaluated by reculturing the cells, and was detectable with concentrations of 200 μg/ml RB or 2000 jig/ml EB; (3) The PDT effect was evaluated using concentrations of 1 to 25 μg/ml of RB and 1 to 2000 μg/ml of EB. The minimum parameters for PDT of cultured LEC were 0.5 μg/ml of RB and 10 μg/ml of EB with a 60 second 0.5 W/cm2 irradiation using a 514.5 nm Argon laser. To mimic the in vivo setting, LEC attached to the anterior lens capsule were incubated with EB to evaluate dye uptake. The minimum parameters were 100 μg/ml and 7 minute of exposure. RB was found most effective and may be useful, but EB was found safer and has potential for conjugation with LEC antibodies for the prevention of secondary cataracts.

Original languageEnglish (US)
Pages (from-to)323-327
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Jun 24 1993
EventOphthalmic Technologies III 1993 - Los Angeles, United States
Duration: Jan 17 1993Jan 22 1993

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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