Comparative phospholipid profiles of control and glaucomatous human trabecular meshwork

Katyayini Aribindi, Yenifer Guerra, Richard K Lee, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

PURPOSE. We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors. METHODS. Control TM and most primary open angle glaucoma (POAG) TM were collected from cadaver donors. A select subset of POAG surgical TM samples also were collected for analyses. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford method, and for select samples confirmed with densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Max triple quadrupole mass spectrometer with precursor ion scan (PIS) or neutral ion loss scan (NLS), using appropriate class specific lipid standards. RESULTS. The comparative profiles of phosphatidylcholine, phosphatidylserine, phosphoethanolamine, and phosphatidylinositol between control and glaucomatous TM showed several species common between them. A number of unique lipids in all four phospholipid classes also were identified in control TM that were absent in glaucoma TM and vice versa. CONCLUSIONS. A number of phospholipids were found to be uniquely present in control but absent in glaucomatous TM and vice versa. Compared to a previous study of control and POAG blood, a number of these phospholipids are absent locally (TM), as well as systemically (in blood).

Original languageEnglish
Pages (from-to)3037-3044
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume54
Issue number4
DOIs
StatePublished - Apr 4 2013

Fingerprint

Trabecular Meshwork
Phosphatidylserines
Phosphatidylinositols
Phosphatidylcholines
Lipids
Phospholipids
Ions
phosphatidylethanolamine
Densitometry
Cadaver
Glaucoma
Gels

Keywords

  • Glaucoma
  • Lipidomics
  • Mass spectrometry
  • Phospholipids
  • Trabecular meshwork

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Comparative phospholipid profiles of control and glaucomatous human trabecular meshwork. / Aribindi, Katyayini; Guerra, Yenifer; Lee, Richard K; Bhattacharya, Sanjoy K.

In: Investigative Ophthalmology and Visual Science, Vol. 54, No. 4, 04.04.2013, p. 3037-3044.

Research output: Contribution to journalArticle

@article{949b1617f3574b8a9fcf46c5fd176fb8,
title = "Comparative phospholipid profiles of control and glaucomatous human trabecular meshwork",
abstract = "PURPOSE. We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors. METHODS. Control TM and most primary open angle glaucoma (POAG) TM were collected from cadaver donors. A select subset of POAG surgical TM samples also were collected for analyses. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford method, and for select samples confirmed with densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Max triple quadrupole mass spectrometer with precursor ion scan (PIS) or neutral ion loss scan (NLS), using appropriate class specific lipid standards. RESULTS. The comparative profiles of phosphatidylcholine, phosphatidylserine, phosphoethanolamine, and phosphatidylinositol between control and glaucomatous TM showed several species common between them. A number of unique lipids in all four phospholipid classes also were identified in control TM that were absent in glaucoma TM and vice versa. CONCLUSIONS. A number of phospholipids were found to be uniquely present in control but absent in glaucomatous TM and vice versa. Compared to a previous study of control and POAG blood, a number of these phospholipids are absent locally (TM), as well as systemically (in blood).",
keywords = "Glaucoma, Lipidomics, Mass spectrometry, Phospholipids, Trabecular meshwork",
author = "Katyayini Aribindi and Yenifer Guerra and Lee, {Richard K} and Bhattacharya, {Sanjoy K}",
year = "2013",
month = "4",
day = "4",
doi = "10.1167/iovs.12-10517",
language = "English",
volume = "54",
pages = "3037--3044",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "4",

}

TY - JOUR

T1 - Comparative phospholipid profiles of control and glaucomatous human trabecular meshwork

AU - Aribindi, Katyayini

AU - Guerra, Yenifer

AU - Lee, Richard K

AU - Bhattacharya, Sanjoy K

PY - 2013/4/4

Y1 - 2013/4/4

N2 - PURPOSE. We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors. METHODS. Control TM and most primary open angle glaucoma (POAG) TM were collected from cadaver donors. A select subset of POAG surgical TM samples also were collected for analyses. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford method, and for select samples confirmed with densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Max triple quadrupole mass spectrometer with precursor ion scan (PIS) or neutral ion loss scan (NLS), using appropriate class specific lipid standards. RESULTS. The comparative profiles of phosphatidylcholine, phosphatidylserine, phosphoethanolamine, and phosphatidylinositol between control and glaucomatous TM showed several species common between them. A number of unique lipids in all four phospholipid classes also were identified in control TM that were absent in glaucoma TM and vice versa. CONCLUSIONS. A number of phospholipids were found to be uniquely present in control but absent in glaucomatous TM and vice versa. Compared to a previous study of control and POAG blood, a number of these phospholipids are absent locally (TM), as well as systemically (in blood).

AB - PURPOSE. We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors. METHODS. Control TM and most primary open angle glaucoma (POAG) TM were collected from cadaver donors. A select subset of POAG surgical TM samples also were collected for analyses. Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford method, and for select samples confirmed with densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Max triple quadrupole mass spectrometer with precursor ion scan (PIS) or neutral ion loss scan (NLS), using appropriate class specific lipid standards. RESULTS. The comparative profiles of phosphatidylcholine, phosphatidylserine, phosphoethanolamine, and phosphatidylinositol between control and glaucomatous TM showed several species common between them. A number of unique lipids in all four phospholipid classes also were identified in control TM that were absent in glaucoma TM and vice versa. CONCLUSIONS. A number of phospholipids were found to be uniquely present in control but absent in glaucomatous TM and vice versa. Compared to a previous study of control and POAG blood, a number of these phospholipids are absent locally (TM), as well as systemically (in blood).

KW - Glaucoma

KW - Lipidomics

KW - Mass spectrometry

KW - Phospholipids

KW - Trabecular meshwork

UR - http://www.scopus.com/inward/record.url?scp=84877088534&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877088534&partnerID=8YFLogxK

U2 - 10.1167/iovs.12-10517

DO - 10.1167/iovs.12-10517

M3 - Article

C2 - 23557733

AN - SCOPUS:84877088534

VL - 54

SP - 3037

EP - 3044

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 4

ER -