Comparative investigation of copper tolerance and identification of putative tolerance related genes in tardigrades

Thomas L. Hygum, Dannie Fobian, Maria Kamilari, Aslak Jørgensen, Morten Schiøtt, Martin Grosell, Nadja Møbjerg

Research output: Contribution to journalArticle

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Abstract

Tardigrades are microscopic aquatic animals renowned for their tolerance toward extreme environmental conditions. The current study is the first to investigate their tolerance toward heavy metals and we present a novel tardigrade toxicant tolerance assay based on activity assessments as a measure of survival. Specifically, we compare tolerance toward copper in four species representing different evolutionary lineages, habitats and adaptation strategies, i.e., a marine heterotardigrade, Echiniscoides sigismundi, a limno-terrestrial heterotardigrade, Echiniscus testudo, a limno-terrestrial eutardigrade, Ramazzottius oberhaeuseri, and a marine eutardigrade, Halobiotus crispae. The latter was sampled at a time of year, when the population is predominantly represented by aberrant P1 cysts, while the other species were in normal active states prior to exposure. Based on volume measurements and a general relation between body mass and copper tolerance, expected tardigrade EC50 values were estimated at 0.5-2 μg l-1. Following 24 h of exposure, tolerance was high with no apparent link to lineage or habitat. EC50s (95% CI), 24 h after exposure, were estimated at 178 (168-186) and 310 (295-328) μg l-1, respectively, for E. sigismundi and R. oberhaeuseri, whereas E. testudo and H. crispae were less affected. Highest tolerance was observed in H. crispae with a mean ± s.e.m. activity of 77 ± 2% (n = 3) 24 h after removal from ~3 mg l-1 copper, suggesting that tardigrade cysts have increased tolerance toward toxicants. In order to identify putative tolerance related genes, an E. sigismundi transcriptome was searched for key enzymes involved in osmoregulation, antioxidant defense and copper metabolism. We found high expression of Na/K ATPase and carbonic anhydrase, known targets for copper. Our transcriptome, furthermore, revealed high expression of antioxidant enzymes, copper transporters, ATOX1, and a Cu-ATPase. In summary, our results indicate that tardigrades express well-known key osmoregulatory enzymes, supporting the hypothesis that copper inhibits sodium turnover as demonstrated for other aquatic organisms. Tardigrades, nevertheless, have high tolerance toward the toxicant, which is likely linked to high expression of antioxidant enzymes and an ability to enter dormant states. Tardigrades, furthermore, seem to have a well-developed battery of cuproproteins involved in copper homeostasis, providing basis for active copper sequestering and excretion.

Original languageEnglish (US)
Article number95
JournalFrontiers in Physiology
Volume8
Issue numberFEB
DOIs
StatePublished - Feb 28 2017

Fingerprint

Copper
Genes
Antioxidants
Enzymes
Transcriptome
Ecosystem
Cysts
Osmoregulation
Aquatic Organisms
Carbonic Anhydrases
Heavy Metals
Adenosine Triphosphatases
Homeostasis
Sodium
Population

Keywords

  • Chemobiosis
  • Copper tolerance
  • Cyst
  • Osmoregulation
  • Stress tolerance
  • Tardigrades
  • Toxicants
  • Transcriptome

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Comparative investigation of copper tolerance and identification of putative tolerance related genes in tardigrades. / Hygum, Thomas L.; Fobian, Dannie; Kamilari, Maria; Jørgensen, Aslak; Schiøtt, Morten; Grosell, Martin; Møbjerg, Nadja.

In: Frontiers in Physiology, Vol. 8, No. FEB, 95, 28.02.2017.

Research output: Contribution to journalArticle

Hygum, Thomas L. ; Fobian, Dannie ; Kamilari, Maria ; Jørgensen, Aslak ; Schiøtt, Morten ; Grosell, Martin ; Møbjerg, Nadja. / Comparative investigation of copper tolerance and identification of putative tolerance related genes in tardigrades. In: Frontiers in Physiology. 2017 ; Vol. 8, No. FEB.
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