Abstract
Objective: To evaluate the most effective intravitreal antibiotic treatment of vancomycin-sensitive and -resistant Enterococcus faecalis endophthalmitis. Design: Animal experiment. Setting: Seventy-eight New Zealand white rabbits received an intravitreal injection of 105 vancomycin- sensitive or -resistant E faecalis organisms in one eye. Infections were allowed to proceed 3 hours before dividing animals randomly into the following treatment groups (n=6, each): the vancomycin-sensitive E faecalis model-(1) vancomycin (1 mg/0.1 mL), (2) combined vancomycin (1 mg/0.1 mL) and amikacin (0.4 mg/0.1 mL), (3) combined vancomycin (1 mg/0.1 mL) and gentamicin (0.1 mg/0.1 mL), (4) combined vancomycin (1 mg/0.1 mL) and ceftazidime (2 mg/0.1 mL), (5) combined ampicillin (5 mg/0.1 mL) and gentamicin (0.1 mg/0.1 mL), and (6) pristinamycin (1 mg/0.1 mL); and the vancomycin-resistant E faecalis model-(1) same as above, excluding group 4. Control groups received sterile balanced salt solution. Twenty-four hours following intravitreal treatment, vitreous humor was collected for quantitative bacteriological studies. Results: Intravitreal therapy with combined vancomycin and amikacin provided the most effective reduction of vancomycin-sensitive E faecalis organisms compared with combined vancomycin and gentamicin therapy (P=.10, Wilcoxon's rank sum test) or any other treatment group (P<.01, Wilcoxon's rank sum test). For vancomycin-resistant E faecalis endophthalmitis model, the combined ampicillin and gentamicin therapy was the most effective, followed by the combined vancomycin and amikacin therapy (P<.01, Wilcoxon's rank sum test). Conclusions: Treatment with intravitreal vancomycin plus amikacin and with intravitreal ampicillin plus gentamicin provide an effective bactericidal therapy for severe experimental vancomycin-sensitive and -resistant E faecalis endophthalmitis, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 61-65 |
| Number of pages | 5 |
| Journal | Archives of Ophthalmology |
| Volume | 114 |
| Issue number | 1 |
| State | Published - Jan 1 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Comparative intravitreal antibiotic therapy for experimental Enterococcus faecalis endophthalmitis. / Choi, Sangkyung; Hahn, Tae Won; Osterhout, Gerard; O'Brien, Terrence.
In: Archives of Ophthalmology, Vol. 114, No. 1, 01.01.1996, p. 61-65.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative intravitreal antibiotic therapy for experimental Enterococcus faecalis endophthalmitis
AU - Choi, Sangkyung
AU - Hahn, Tae Won
AU - Osterhout, Gerard
AU - O'Brien, Terrence
PY - 1996/1/1
Y1 - 1996/1/1
N2 - Objective: To evaluate the most effective intravitreal antibiotic treatment of vancomycin-sensitive and -resistant Enterococcus faecalis endophthalmitis. Design: Animal experiment. Setting: Seventy-eight New Zealand white rabbits received an intravitreal injection of 105 vancomycin- sensitive or -resistant E faecalis organisms in one eye. Infections were allowed to proceed 3 hours before dividing animals randomly into the following treatment groups (n=6, each): the vancomycin-sensitive E faecalis model-(1) vancomycin (1 mg/0.1 mL), (2) combined vancomycin (1 mg/0.1 mL) and amikacin (0.4 mg/0.1 mL), (3) combined vancomycin (1 mg/0.1 mL) and gentamicin (0.1 mg/0.1 mL), (4) combined vancomycin (1 mg/0.1 mL) and ceftazidime (2 mg/0.1 mL), (5) combined ampicillin (5 mg/0.1 mL) and gentamicin (0.1 mg/0.1 mL), and (6) pristinamycin (1 mg/0.1 mL); and the vancomycin-resistant E faecalis model-(1) same as above, excluding group 4. Control groups received sterile balanced salt solution. Twenty-four hours following intravitreal treatment, vitreous humor was collected for quantitative bacteriological studies. Results: Intravitreal therapy with combined vancomycin and amikacin provided the most effective reduction of vancomycin-sensitive E faecalis organisms compared with combined vancomycin and gentamicin therapy (P=.10, Wilcoxon's rank sum test) or any other treatment group (P<.01, Wilcoxon's rank sum test). For vancomycin-resistant E faecalis endophthalmitis model, the combined ampicillin and gentamicin therapy was the most effective, followed by the combined vancomycin and amikacin therapy (P<.01, Wilcoxon's rank sum test). Conclusions: Treatment with intravitreal vancomycin plus amikacin and with intravitreal ampicillin plus gentamicin provide an effective bactericidal therapy for severe experimental vancomycin-sensitive and -resistant E faecalis endophthalmitis, respectively.
AB - Objective: To evaluate the most effective intravitreal antibiotic treatment of vancomycin-sensitive and -resistant Enterococcus faecalis endophthalmitis. Design: Animal experiment. Setting: Seventy-eight New Zealand white rabbits received an intravitreal injection of 105 vancomycin- sensitive or -resistant E faecalis organisms in one eye. Infections were allowed to proceed 3 hours before dividing animals randomly into the following treatment groups (n=6, each): the vancomycin-sensitive E faecalis model-(1) vancomycin (1 mg/0.1 mL), (2) combined vancomycin (1 mg/0.1 mL) and amikacin (0.4 mg/0.1 mL), (3) combined vancomycin (1 mg/0.1 mL) and gentamicin (0.1 mg/0.1 mL), (4) combined vancomycin (1 mg/0.1 mL) and ceftazidime (2 mg/0.1 mL), (5) combined ampicillin (5 mg/0.1 mL) and gentamicin (0.1 mg/0.1 mL), and (6) pristinamycin (1 mg/0.1 mL); and the vancomycin-resistant E faecalis model-(1) same as above, excluding group 4. Control groups received sterile balanced salt solution. Twenty-four hours following intravitreal treatment, vitreous humor was collected for quantitative bacteriological studies. Results: Intravitreal therapy with combined vancomycin and amikacin provided the most effective reduction of vancomycin-sensitive E faecalis organisms compared with combined vancomycin and gentamicin therapy (P=.10, Wilcoxon's rank sum test) or any other treatment group (P<.01, Wilcoxon's rank sum test). For vancomycin-resistant E faecalis endophthalmitis model, the combined ampicillin and gentamicin therapy was the most effective, followed by the combined vancomycin and amikacin therapy (P<.01, Wilcoxon's rank sum test). Conclusions: Treatment with intravitreal vancomycin plus amikacin and with intravitreal ampicillin plus gentamicin provide an effective bactericidal therapy for severe experimental vancomycin-sensitive and -resistant E faecalis endophthalmitis, respectively.
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UR - http://www.scopus.com/inward/citedby.url?scp=9044237690&partnerID=8YFLogxK
M3 - Article
C2 - 8540852
AN - SCOPUS:9044237690
VL - 114
SP - 61
EP - 65
JO - JAMA Ophthalmology
JF - JAMA Ophthalmology
SN - 2168-6165
IS - 1
ER -