Comparative histopathologic consequences of photothrombotic occlusion of the distal middle cerebral artery in sprague-dawley and wistar rats

Carrie G. Markgraf, Susan Kraydieh, Ricardo Prado, Brant D. Watson, W. Dalton Dietrich, Myron Ginsberg

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Background and Purpose: We have developed a minimally invasive model of photothrombotic occlusion of the distal middle cerebral artery in rats and have evaluated the patterns and features of the resulting histopathologic injury in two normotensive strains. Methods: Food-deprived male Sprague-Dawley (n=14) and Wistar (n=10) rats anesthetized with halothane/nitrous oxide underwent a small craniotomy to expose the right distal middle cerebral artery just above the rhinal fissure. The animals were injected intravenously with the photosensitizing dye rose bengal, and the distal middle cerebral artery was irradiated with light from an argon laser-activated dye laser at three separate points to induce thrombotic occlusion. The ipsilateral common carotid artery was then permanently occluded, and the contralateral common carotid artery was occluded for 60 minutes. Three days later, the brains were perfusion-fixed and prepared for histopathologic examination, and infarct volume was determined by quantitative planimetry. Results: In Sprague-Dawley rats, a large consistent temporoparietal cortical infarct was observed; mean±SD infarct volume was 130.5±40.0 mm3 (coefficient of variation, 30.7%) and a relatively small adjacent zone of selective neuronal necrosis ("incomplete infarction"), amounting to only 9.1% of the total injury volume, was also seen. By contrast, Wistar rats had smaller and more variable cortical infarcts (volume, 48.4±26.9 mm3; coefficient of variation, 55.6%) but displayed a much more substantial zone of incomplete cortical infarction (volume, 20.8±10.1 mm3; 30.1% of the total injury volume). In neither strain was infarct size related to alterations of blood pressure. In both strains, infarcts were limited to the cortex, typically involving the parietal cortex, somatosensory cortex, and forelimb region. Three rats exhibited infarcts in the contralateral hemisphere. Conclusions: This model has the advantages of necessitating only minimal surgery, allowing the dura to remain intact, and avoiding mechanical trauma to the brain surface. In Sprague-Dawley rats, the resulting large cortical infarct exhibited relatively small interanimal variation, making the model suitable, for example, for replicate studies of pharmacotherapy. In Wistar rats, the large zone of incomplete infarction, a unique feature heretofore undescribed in rodent models of permanent focal ischemia, lends the model to the study of the pathomechanisms underlying graded cortical ischemic injury.

Original languageEnglish
Pages (from-to)286-292
Number of pages7
JournalStroke
Volume24
Issue number2
StatePublished - Feb 1 1993

Fingerprint

Middle Cerebral Artery Infarction
Sprague Dawley Rats
Wistar Rats
Infarction
Common Carotid Artery
Middle Cerebral Artery
Wounds and Injuries
Dye Lasers
Rose Bengal
Parietal Lobe
Somatosensory Cortex
Forelimb
Argon
Craniotomy
Nitrous Oxide
Halothane
Nose
Rodentia
Lasers
Necrosis

Keywords

  • Animal models
  • Cerebral arteries
  • Rats
  • Thrombosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Neuroscience(all)

Cite this

Comparative histopathologic consequences of photothrombotic occlusion of the distal middle cerebral artery in sprague-dawley and wistar rats. / Markgraf, Carrie G.; Kraydieh, Susan; Prado, Ricardo; Watson, Brant D.; Dalton Dietrich, W.; Ginsberg, Myron.

In: Stroke, Vol. 24, No. 2, 01.02.1993, p. 286-292.

Research output: Contribution to journalArticle

@article{d5dd82f3f54c47cda1b8fca4e7aaaf79,
title = "Comparative histopathologic consequences of photothrombotic occlusion of the distal middle cerebral artery in sprague-dawley and wistar rats",
abstract = "Background and Purpose: We have developed a minimally invasive model of photothrombotic occlusion of the distal middle cerebral artery in rats and have evaluated the patterns and features of the resulting histopathologic injury in two normotensive strains. Methods: Food-deprived male Sprague-Dawley (n=14) and Wistar (n=10) rats anesthetized with halothane/nitrous oxide underwent a small craniotomy to expose the right distal middle cerebral artery just above the rhinal fissure. The animals were injected intravenously with the photosensitizing dye rose bengal, and the distal middle cerebral artery was irradiated with light from an argon laser-activated dye laser at three separate points to induce thrombotic occlusion. The ipsilateral common carotid artery was then permanently occluded, and the contralateral common carotid artery was occluded for 60 minutes. Three days later, the brains were perfusion-fixed and prepared for histopathologic examination, and infarct volume was determined by quantitative planimetry. Results: In Sprague-Dawley rats, a large consistent temporoparietal cortical infarct was observed; mean±SD infarct volume was 130.5±40.0 mm3 (coefficient of variation, 30.7{\%}) and a relatively small adjacent zone of selective neuronal necrosis ({"}incomplete infarction{"}), amounting to only 9.1{\%} of the total injury volume, was also seen. By contrast, Wistar rats had smaller and more variable cortical infarcts (volume, 48.4±26.9 mm3; coefficient of variation, 55.6{\%}) but displayed a much more substantial zone of incomplete cortical infarction (volume, 20.8±10.1 mm3; 30.1{\%} of the total injury volume). In neither strain was infarct size related to alterations of blood pressure. In both strains, infarcts were limited to the cortex, typically involving the parietal cortex, somatosensory cortex, and forelimb region. Three rats exhibited infarcts in the contralateral hemisphere. Conclusions: This model has the advantages of necessitating only minimal surgery, allowing the dura to remain intact, and avoiding mechanical trauma to the brain surface. In Sprague-Dawley rats, the resulting large cortical infarct exhibited relatively small interanimal variation, making the model suitable, for example, for replicate studies of pharmacotherapy. In Wistar rats, the large zone of incomplete infarction, a unique feature heretofore undescribed in rodent models of permanent focal ischemia, lends the model to the study of the pathomechanisms underlying graded cortical ischemic injury.",
keywords = "Animal models, Cerebral arteries, Rats, Thrombosis",
author = "Markgraf, {Carrie G.} and Susan Kraydieh and Ricardo Prado and Watson, {Brant D.} and {Dalton Dietrich}, W. and Myron Ginsberg",
year = "1993",
month = "2",
day = "1",
language = "English",
volume = "24",
pages = "286--292",
journal = "Stroke",
issn = "0039-2499",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Comparative histopathologic consequences of photothrombotic occlusion of the distal middle cerebral artery in sprague-dawley and wistar rats

AU - Markgraf, Carrie G.

AU - Kraydieh, Susan

AU - Prado, Ricardo

AU - Watson, Brant D.

AU - Dalton Dietrich, W.

AU - Ginsberg, Myron

PY - 1993/2/1

Y1 - 1993/2/1

N2 - Background and Purpose: We have developed a minimally invasive model of photothrombotic occlusion of the distal middle cerebral artery in rats and have evaluated the patterns and features of the resulting histopathologic injury in two normotensive strains. Methods: Food-deprived male Sprague-Dawley (n=14) and Wistar (n=10) rats anesthetized with halothane/nitrous oxide underwent a small craniotomy to expose the right distal middle cerebral artery just above the rhinal fissure. The animals were injected intravenously with the photosensitizing dye rose bengal, and the distal middle cerebral artery was irradiated with light from an argon laser-activated dye laser at three separate points to induce thrombotic occlusion. The ipsilateral common carotid artery was then permanently occluded, and the contralateral common carotid artery was occluded for 60 minutes. Three days later, the brains were perfusion-fixed and prepared for histopathologic examination, and infarct volume was determined by quantitative planimetry. Results: In Sprague-Dawley rats, a large consistent temporoparietal cortical infarct was observed; mean±SD infarct volume was 130.5±40.0 mm3 (coefficient of variation, 30.7%) and a relatively small adjacent zone of selective neuronal necrosis ("incomplete infarction"), amounting to only 9.1% of the total injury volume, was also seen. By contrast, Wistar rats had smaller and more variable cortical infarcts (volume, 48.4±26.9 mm3; coefficient of variation, 55.6%) but displayed a much more substantial zone of incomplete cortical infarction (volume, 20.8±10.1 mm3; 30.1% of the total injury volume). In neither strain was infarct size related to alterations of blood pressure. In both strains, infarcts were limited to the cortex, typically involving the parietal cortex, somatosensory cortex, and forelimb region. Three rats exhibited infarcts in the contralateral hemisphere. Conclusions: This model has the advantages of necessitating only minimal surgery, allowing the dura to remain intact, and avoiding mechanical trauma to the brain surface. In Sprague-Dawley rats, the resulting large cortical infarct exhibited relatively small interanimal variation, making the model suitable, for example, for replicate studies of pharmacotherapy. In Wistar rats, the large zone of incomplete infarction, a unique feature heretofore undescribed in rodent models of permanent focal ischemia, lends the model to the study of the pathomechanisms underlying graded cortical ischemic injury.

AB - Background and Purpose: We have developed a minimally invasive model of photothrombotic occlusion of the distal middle cerebral artery in rats and have evaluated the patterns and features of the resulting histopathologic injury in two normotensive strains. Methods: Food-deprived male Sprague-Dawley (n=14) and Wistar (n=10) rats anesthetized with halothane/nitrous oxide underwent a small craniotomy to expose the right distal middle cerebral artery just above the rhinal fissure. The animals were injected intravenously with the photosensitizing dye rose bengal, and the distal middle cerebral artery was irradiated with light from an argon laser-activated dye laser at three separate points to induce thrombotic occlusion. The ipsilateral common carotid artery was then permanently occluded, and the contralateral common carotid artery was occluded for 60 minutes. Three days later, the brains were perfusion-fixed and prepared for histopathologic examination, and infarct volume was determined by quantitative planimetry. Results: In Sprague-Dawley rats, a large consistent temporoparietal cortical infarct was observed; mean±SD infarct volume was 130.5±40.0 mm3 (coefficient of variation, 30.7%) and a relatively small adjacent zone of selective neuronal necrosis ("incomplete infarction"), amounting to only 9.1% of the total injury volume, was also seen. By contrast, Wistar rats had smaller and more variable cortical infarcts (volume, 48.4±26.9 mm3; coefficient of variation, 55.6%) but displayed a much more substantial zone of incomplete cortical infarction (volume, 20.8±10.1 mm3; 30.1% of the total injury volume). In neither strain was infarct size related to alterations of blood pressure. In both strains, infarcts were limited to the cortex, typically involving the parietal cortex, somatosensory cortex, and forelimb region. Three rats exhibited infarcts in the contralateral hemisphere. Conclusions: This model has the advantages of necessitating only minimal surgery, allowing the dura to remain intact, and avoiding mechanical trauma to the brain surface. In Sprague-Dawley rats, the resulting large cortical infarct exhibited relatively small interanimal variation, making the model suitable, for example, for replicate studies of pharmacotherapy. In Wistar rats, the large zone of incomplete infarction, a unique feature heretofore undescribed in rodent models of permanent focal ischemia, lends the model to the study of the pathomechanisms underlying graded cortical ischemic injury.

KW - Animal models

KW - Cerebral arteries

KW - Rats

KW - Thrombosis

UR - http://www.scopus.com/inward/record.url?scp=0027463828&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027463828&partnerID=8YFLogxK

M3 - Article

VL - 24

SP - 286

EP - 292

JO - Stroke

JF - Stroke

SN - 0039-2499

IS - 2

ER -