Comparative genomic hybridization in childhood acute lymphoblastic leukemia

M. L. Larramendy, T. Huhta, K. Vettenranta, W. El-Rifai, J. Lundin, S. Pakkala, U. M. Saarinen-Pihkala, S. Knuutila

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26 Scopus citations

Abstract

DNA copy number changes were studied by comparative genomic hybridization (CGH) on bone marrow samples obtained from 72 patients with childhood acute lymphoblastic leukemia (ALL) at diagnosis. The patients had been admitted to the Helsinki University Central Hospital (Finland) between 1982 and 1997. CGH showed DNA copy number changes in 45 patients (62.5%) with a mean of 4.6 aberrations per patient (range, 1 to 22). The results of CGH and chromosome banding analysis were generally concordant, but CGH facilitated specific karyotyping in 34 cases. DNA copy number gains were more frequent than losses (gains:losses, 6:1). Gains of DNA sequences affected almost exclusively whole chromosomes and were most commonly observed in chromosomes 21 (25%), 18 (22.2%), X (19.4%), 10 (19.4%) and 17 (19.4%). The most common partial gain was 1q31-q32 (8.3%). The most common gains of chromosomes 21, 18, X, 10, 17, 14, 4, 6 and 8 appeared concurrently. High-level amplifications of small chromosome regions were sporadic, detected only in two patients (2.8%). Chromosome 21 was involved in both cases. The most common losses were 9p22-pter (12.5%) and 12p13-pter (11.1%). No statistically significant association between the CGH findings and the diagnostic white blood cell count was observed.

Original languageEnglish (US)
Pages (from-to)1638-1644
Number of pages7
JournalLeukemia
Volume12
Issue number10
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Keywords

  • Acute lymphoblastic leukemia
  • Comparative genomic hybridization
  • DNA copy number changes
  • Gains
  • Losses

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Larramendy, M. L., Huhta, T., Vettenranta, K., El-Rifai, W., Lundin, J., Pakkala, S., Saarinen-Pihkala, U. M., & Knuutila, S. (1998). Comparative genomic hybridization in childhood acute lymphoblastic leukemia. Leukemia, 12(10), 1638-1644. https://doi.org/10.1038/sj.leu.2401142