Comparative genomic hybridization in childhood acute lymphoblastic leukemia

M. L. Larramendy, T. Huhta, K. Vettenranta, Wael El-Rifai, J. Lundin, S. Pakkala, U. M. Saarinen-Pihkala, S. Knuutila

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

DNA copy number changes were studied by comparative genomic hybridization (CGH) on bone marrow samples obtained from 72 patients with childhood acute lymphoblastic leukemia (ALL) at diagnosis. The patients had been admitted to the Helsinki University Central Hospital (Finland) between 1982 and 1997. CGH showed DNA copy number changes in 45 patients (62.5%) with a mean of 4.6 aberrations per patient (range, 1 to 22). The results of CGH and chromosome banding analysis were generally concordant, but CGH facilitated specific karyotyping in 34 cases. DNA copy number gains were more frequent than losses (gains:losses, 6:1). Gains of DNA sequences affected almost exclusively whole chromosomes and were most commonly observed in chromosomes 21 (25%), 18 (22.2%), X (19.4%), 10 (19.4%) and 17 (19.4%). The most common partial gain was 1q31-q32 (8.3%). The most common gains of chromosomes 21, 18, X, 10, 17, 14, 4, 6 and 8 appeared concurrently. High-level amplifications of small chromosome regions were sporadic, detected only in two patients (2.8%). Chromosome 21 was involved in both cases. The most common losses were 9p22-pter (12.5%) and 12p13-pter (11.1%). No statistically significant association between the CGH findings and the diagnostic white blood cell count was observed.

Original languageEnglish (US)
Pages (from-to)1638-1644
Number of pages7
JournalLeukemia
Volume12
Issue number10
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Comparative Genomic Hybridization
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Chromosomes, Human, Pair 21
DNA Copy Number Variations
Chromosomes
Chromosome Banding
Chromosomes, Human, Pair 18
Karyotyping
Finland
Leukocyte Count
Bone Marrow
DNA

Keywords

  • Acute lymphoblastic leukemia
  • Comparative genomic hybridization
  • DNA copy number changes
  • Gains
  • Losses

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Larramendy, M. L., Huhta, T., Vettenranta, K., El-Rifai, W., Lundin, J., Pakkala, S., ... Knuutila, S. (1998). Comparative genomic hybridization in childhood acute lymphoblastic leukemia. Leukemia, 12(10), 1638-1644. https://doi.org/10.1038/sj.leu.2401142

Comparative genomic hybridization in childhood acute lymphoblastic leukemia. / Larramendy, M. L.; Huhta, T.; Vettenranta, K.; El-Rifai, Wael; Lundin, J.; Pakkala, S.; Saarinen-Pihkala, U. M.; Knuutila, S.

In: Leukemia, Vol. 12, No. 10, 01.01.1998, p. 1638-1644.

Research output: Contribution to journalArticle

Larramendy, ML, Huhta, T, Vettenranta, K, El-Rifai, W, Lundin, J, Pakkala, S, Saarinen-Pihkala, UM & Knuutila, S 1998, 'Comparative genomic hybridization in childhood acute lymphoblastic leukemia', Leukemia, vol. 12, no. 10, pp. 1638-1644. https://doi.org/10.1038/sj.leu.2401142
Larramendy ML, Huhta T, Vettenranta K, El-Rifai W, Lundin J, Pakkala S et al. Comparative genomic hybridization in childhood acute lymphoblastic leukemia. Leukemia. 1998 Jan 1;12(10):1638-1644. https://doi.org/10.1038/sj.leu.2401142
Larramendy, M. L. ; Huhta, T. ; Vettenranta, K. ; El-Rifai, Wael ; Lundin, J. ; Pakkala, S. ; Saarinen-Pihkala, U. M. ; Knuutila, S. / Comparative genomic hybridization in childhood acute lymphoblastic leukemia. In: Leukemia. 1998 ; Vol. 12, No. 10. pp. 1638-1644.
@article{d64ef542741c42a6bc94caf99b4336bd,
title = "Comparative genomic hybridization in childhood acute lymphoblastic leukemia",
abstract = "DNA copy number changes were studied by comparative genomic hybridization (CGH) on bone marrow samples obtained from 72 patients with childhood acute lymphoblastic leukemia (ALL) at diagnosis. The patients had been admitted to the Helsinki University Central Hospital (Finland) between 1982 and 1997. CGH showed DNA copy number changes in 45 patients (62.5{\%}) with a mean of 4.6 aberrations per patient (range, 1 to 22). The results of CGH and chromosome banding analysis were generally concordant, but CGH facilitated specific karyotyping in 34 cases. DNA copy number gains were more frequent than losses (gains:losses, 6:1). Gains of DNA sequences affected almost exclusively whole chromosomes and were most commonly observed in chromosomes 21 (25{\%}), 18 (22.2{\%}), X (19.4{\%}), 10 (19.4{\%}) and 17 (19.4{\%}). The most common partial gain was 1q31-q32 (8.3{\%}). The most common gains of chromosomes 21, 18, X, 10, 17, 14, 4, 6 and 8 appeared concurrently. High-level amplifications of small chromosome regions were sporadic, detected only in two patients (2.8{\%}). Chromosome 21 was involved in both cases. The most common losses were 9p22-pter (12.5{\%}) and 12p13-pter (11.1{\%}). No statistically significant association between the CGH findings and the diagnostic white blood cell count was observed.",
keywords = "Acute lymphoblastic leukemia, Comparative genomic hybridization, DNA copy number changes, Gains, Losses",
author = "Larramendy, {M. L.} and T. Huhta and K. Vettenranta and Wael El-Rifai and J. Lundin and S. Pakkala and Saarinen-Pihkala, {U. M.} and S. Knuutila",
year = "1998",
month = "1",
day = "1",
doi = "10.1038/sj.leu.2401142",
language = "English (US)",
volume = "12",
pages = "1638--1644",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Comparative genomic hybridization in childhood acute lymphoblastic leukemia

AU - Larramendy, M. L.

AU - Huhta, T.

AU - Vettenranta, K.

AU - El-Rifai, Wael

AU - Lundin, J.

AU - Pakkala, S.

AU - Saarinen-Pihkala, U. M.

AU - Knuutila, S.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - DNA copy number changes were studied by comparative genomic hybridization (CGH) on bone marrow samples obtained from 72 patients with childhood acute lymphoblastic leukemia (ALL) at diagnosis. The patients had been admitted to the Helsinki University Central Hospital (Finland) between 1982 and 1997. CGH showed DNA copy number changes in 45 patients (62.5%) with a mean of 4.6 aberrations per patient (range, 1 to 22). The results of CGH and chromosome banding analysis were generally concordant, but CGH facilitated specific karyotyping in 34 cases. DNA copy number gains were more frequent than losses (gains:losses, 6:1). Gains of DNA sequences affected almost exclusively whole chromosomes and were most commonly observed in chromosomes 21 (25%), 18 (22.2%), X (19.4%), 10 (19.4%) and 17 (19.4%). The most common partial gain was 1q31-q32 (8.3%). The most common gains of chromosomes 21, 18, X, 10, 17, 14, 4, 6 and 8 appeared concurrently. High-level amplifications of small chromosome regions were sporadic, detected only in two patients (2.8%). Chromosome 21 was involved in both cases. The most common losses were 9p22-pter (12.5%) and 12p13-pter (11.1%). No statistically significant association between the CGH findings and the diagnostic white blood cell count was observed.

AB - DNA copy number changes were studied by comparative genomic hybridization (CGH) on bone marrow samples obtained from 72 patients with childhood acute lymphoblastic leukemia (ALL) at diagnosis. The patients had been admitted to the Helsinki University Central Hospital (Finland) between 1982 and 1997. CGH showed DNA copy number changes in 45 patients (62.5%) with a mean of 4.6 aberrations per patient (range, 1 to 22). The results of CGH and chromosome banding analysis were generally concordant, but CGH facilitated specific karyotyping in 34 cases. DNA copy number gains were more frequent than losses (gains:losses, 6:1). Gains of DNA sequences affected almost exclusively whole chromosomes and were most commonly observed in chromosomes 21 (25%), 18 (22.2%), X (19.4%), 10 (19.4%) and 17 (19.4%). The most common partial gain was 1q31-q32 (8.3%). The most common gains of chromosomes 21, 18, X, 10, 17, 14, 4, 6 and 8 appeared concurrently. High-level amplifications of small chromosome regions were sporadic, detected only in two patients (2.8%). Chromosome 21 was involved in both cases. The most common losses were 9p22-pter (12.5%) and 12p13-pter (11.1%). No statistically significant association between the CGH findings and the diagnostic white blood cell count was observed.

KW - Acute lymphoblastic leukemia

KW - Comparative genomic hybridization

KW - DNA copy number changes

KW - Gains

KW - Losses

UR - http://www.scopus.com/inward/record.url?scp=0031689134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031689134&partnerID=8YFLogxK

U2 - 10.1038/sj.leu.2401142

DO - 10.1038/sj.leu.2401142

M3 - Article

VL - 12

SP - 1638

EP - 1644

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 10

ER -