Comparative genome hybridization analysis of laser-capture microdissected in situ melanoma

Vladimir Vincek, Suying Xu, Yao Shan Fan

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Background: The progression of melanoma occurs through discrete stages with known clinical and histologic features. Although many molecular events that occur during the progression of invasive and metastatic melanomas have been elucidated, there is limited knowledge of genetic changes that occur in the earliest stages of melanoma development. In this pilot study, we investigated genetic changes that happen in in situ melanoma so that we can better understand early melanoma development. Materials and methods: DNA was extracted from five laser-capture microdissected Clark's level III melanomas, five in situ melanomas and five compound nevi all from sun exposed skin. Array-based comparative genomic hybridization was performed using Agilent 44 K platform. Results: The group of Clark's level III melanomas was characterized with multiple large deletions and duplications. In the group of in situ melanoma, deletions and duplications were limited in size. Deletions in in situ melanomas were present only on chromosomes 13q and 16q. Compound nevi did not show any significant chromosomal aberrations. Conclusion: In situ melanomas show characteristic chromosomal aberrations that are limited compared to melanomas that invade the dermis. Deletion of 13q found in in situ melanomas, which encompass the Rb1 tumor suppressor gene, might be one of the first events in the development of melanoma. Vincek V, Xu S, Fan Y-S. Comparative genome hybridization analysis of laser-capture microdissected in situ melanoma.

Original languageEnglish (US)
Pages (from-to)3-7
Number of pages5
JournalJournal of Cutaneous Pathology
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2010

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Dermatology

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